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concentrations were determined using ELISA. Heart rate (HR), gastrointestinal temperature (Tgi), physiological strain index (PSI) and changes in blood biomarkers were assessed using 2-way ANOVA and paired Student's t-test with p<0.05 as statistically significant. RESULTS: Participants observed similar baseline Tgi (37.0 ± 0.2ºC; p=0.910) and HR (69 ± 7 bpm; p=0.469). After ingestion at rest, Tgi was lower in ICE (36.5 ± 0.2ºC) compared to AMB (36.9 ± 0.2ºC, p<0.001). Tgi was similar post-FDE (ICE:38.4 ± 0.4ºC, AMB: 38.6 ± 0.3ºC; p=0.196) and post-ECT (both 39.5 ± 0.5ºC; p=0.976). PSI for FDE (ICE: 5.6 ± 0.6, AMB: 5.5 ± 0.7; p=0.652) and ECT (ICE: 6.8 ± 1.0, AMB: 6.9 ± 1.0; p=0.668) were also similar. ECT run times for ICE (48 ± 13 min) and AMB (45 ± 13 min; p=0.142) did not differ. Serum cortisol concentrations were similar at baseline (5.1 ± 2.7 ug/dL, p=0.840) and post-FDE (4.4 ± 3.1 ug/dL, p=0.804). Post-ECT serum cortisol concentrations were lower in ICE (7.8 ± 2.5 ug/dL) than AMB (9.7 ± 3.1 ug/dL, p=0.03). Serum concentrations of i-FABP, LPS and IL-6 were similar at baseline, post-FDE and post-ECT (p>0.05). CONCLUSION: Ice slurry ingestion before and during exercise reduces serum cortisol levels via mechanisms independent from the physiological strain incurred during exercise.