Trichoderma viride is a microorganism that is commonly found in moist soil and can be isolated from the root area of the shallot plant (Allium ascalonicum L.). The study aim was to determine the mechanism and the difference in percentage of inhibitory power by Trichoderma viride in inhibiting growth of Alternaria porri pathogen at pH 4.8 and 7. The descriptive method was used by descriptive method to isolate Alternaria porri and Trichoderma viride fungi and then purified, and the experimental method tested Trichoderma viride fungal antagonists against Alternaria porri with two PDA media treatments pH 4.8 that observed for 3 days and pH 7 observed for 4 days. The results of the percentage of inhibitory power on the day 1,2,3 were 9%, 34.2% and 35% (pH 4.8), and the results of the percentage of inhibition power of the day 1,2,3 and 4 were 1.9%, 29.9%, 35% and 39.4% (pH 7). The inhibition mechanism of Trichoderma viride in inhibiting Alternaria porri fungi through the stages of antibiosis, parasitism and lysis. Keywords: Trichoderma viride, Alternaria porri, shallot plants, pH 4,8 and pH 7. ABSTRAK Jamur Trichoderma viride merupakan mikroorganisme yang umum dijumpai dalam tanah yang lembab dan dapat diisolasi dari daerah perakaran tanaman bawang merah (Allium ascalonicum L.) Penelitian bertujuan untuk mengetahui mekanisme dan perbedaan persentase daya hambat oleh jamur Trichoderma viride dalam menghambat pertumbuhan patogen Alternaria porri pada pH 4,8 dan 7. Metode yamg digunakan metode deskriptif dengan mengisolasi jamur Alternaria porri dan Trichoderma viride lalu dimurnikan, dan metode eksperimen melakukan uji antagonis jamur Trichoderma viride terhadap Alternaria porri dengan dua perlakuan media PDA pH 4,8 yamg diaamati selama 3 hari dan pH 7 yang diamati selama 4 hari. Hasil persentase daya hambat pada hari ke 1,2,3 yaitu 9%, 34,2% dan 35%  (pH 4,8), dan  hasil persentase daya hambat hari ke 1,2,3 dan 4 yaitu 1,9%, 29,9%, 35% dan 39,4% (pH 7). Hal ini diketahui bahwa hasil terakhir persentase daya hambat 35% (pH 4,8) dan 39,4% (pH 7). Mekanisme Penghambatan Jamur Trichoderma viride dalam menghambat jamur Alternaria porri melalui tahap antibiosis, parasitisme dan lisis. Kata kunci: Trichoderma viride, Alternaria porri, tanaman bawang merah, pH 4,8 dan pH 7.

Batu as a tourism city produces a lot of vegetables, flowers, and ornamental plants. Many cut flower waste from sorting is not suitable for sale. Meanwhile, SMK Negeri 2 Batu has developed several products to be marketed and is a place for students to study entrepreneurship. However, there are problems in getting market demand due to intense competition. Therefore, production requires continuous innovation and creativity. This new method is offered to increase grades by teaching them the skills of a modern dry herbarium using waste flowers from the surrounding environment. The purpose of this activity is that the teachers and students of SMK Negeri 2 Batu have the knowledge and skills to create a modern dry herbarium with Oshibana techniques. Teaching is done by giving lectures, demonstrations, practices, and evaluations. The results show increased knowledge to utilize flower and leaf waste through manufacturing activities of modern dried herbarium with the Oshibana technique. The program also provides counseling on how to use wasted flowers and leaves that can be done properly. The training to make a modern dry herbarium which was later named "Beautiful Herbarium" was able to improve the knowledge and skills of participants. The "Beautiful Herbarium" extra-curriculum was held to ensure the sustainability of the program.

The shallots production enhancements, an improvement in cultivation techniques and organic fertilizer is needed. Biogas liquid waste is one of the organic fertilizers that can be used in plants. The benefit of biogas liquid waste is that it can improve soil properties and produce agricultural products that are safe for health. The purpose of this study was to determine the effect of dose and time of bio-slurry fertilizer on the growth of red onion (Allium cepa L.). Research used a randomized block design consisting of two factors. The first factor is the time of fertilizer application which consists of four levels, namely: control, W1, W2 and W3. The second factor is the administration of bio-slurry fertilizer dose of 25 ml, 50 ml, 75 ml and 100 ml. The results showed that the application of bio-slurry fertilizer affected the growth of shallots. The treatment of D4W1 (fertilizer every week with a dose of 100 ml) showed the best results in each parameter, namely plant height, leaf number, leaf area, root length, wet weight and dry weightABSTRAKPeningkatan produksi bawang merah diperlukan adanya perbaikan teknik budidaya  dan pemberian pupuk organik. Limbah cair biogas adalah salah satu pupuk organik yang dapat digunakan pada tanaman. Manfaat limbah cair biogas adalah  dapat memperbaiki sifat-sifat tanah dan menghasilkan produk pertanian yang aman bagi kesehatan. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh dosis dan waktu pemberian pupuk bio-slurry terhadap pertumbuhan bawang merah (Allium cepa L.) Penelitian menggunakan Rancangan Acak Kelompok yang terdiri dari dua faktor. Faktor pertama yaitu waktu pemberian pupuk yang terdiri dari empat taraf yaitu: kontrol, W1, W2 dan W3. Faktor kedua adalah pemberian dosis pupuk bio-slurry yaitu 25 ml, 50ml, 75 ml dan 100 ml. Hasil penelitian menunjukkan bahwa pemberian pupuk bio-slurry berpengaruh terhadap pertumbuhan bawang merah. Perlakuan D4W1 (pemberian pupuk setiap minggu dengan dosis 100 ml).menunjukkan hasil yang terbaik pada setiap parameter yaitu tinggi tanaman, jumlah daun, luas daun, panjang akar, berat basah dan berat kering.Kata kunci: Pupuk Bio-Slurry, Bawang merah (Allium cepa L.), waktu pemberian pupuk

Olive (Olea europaea) have been cultivated and grown well in tropical climates such as Indonesia. Indonesia local community have used olive as herbal medicines due to its active compounds known as oleuropein that has many biological activities including as neurotherapy in Alzheimer's disease. The purpose of this study is to explore the potential phenolic compounds of olive and examine the acetylcholinesterase (AChE) inhibitory activity displayed by different olive polyphenols through a silico approach. The bioactive compounds of olive which had been analyzed in this study were phenolic compound included oleuropein, demethyl-oleuropein, ligstroside, oleoside, verbascoside, luteolin 7-glucoside, and hydroxytyrosol. Interaction of bioactive compounds with acetylcholinesterase (AChE) was analyzed through molecular specific docking using AutoDock Vina with Pyrx Software. The result elucidate that olive contain potential biological activities as antioxidant, anti-inflammatory, antineoplastic, free radical scavenger, antibacterial, antifungal, expression TP53 enhancer, caspase 8 stimulant, platelet adhesion inhibitor, treatment for lipoprotein disorder, antiviral and dementia treatment/Alzheimer disease. The highest bioactivity percentage of olive phenolic compound are as an antioxidant of 82%, anti-inflammatory of 73%, and anti-cancer (antineoplastic) of 70% respectively. Based on molecular docking analysis show one of olive phenolic compound of the dimethyl-oleuropein has strong interaction with AChE as pointed in the binding affinity of demethyl-oleuropein +AChE of-8.9 kcal/mol has closed to galanthamine binding affinity of-10.3 kcal/mol. Along with glutamate acid 202, tyrosine 133 and tyrosine 124 are the major contributors in the target-ligand interactions. The selected demethyl-oleuropein ought to be tested in clinical studies to discover new neuro-therapeutic candidates.

Kesambi (Schleichera oleosa) is a plant belonging to the Sapindaceae familia. This study aims to determine the secondary metabolite compounds found in kesambi leaves through histochemical analysis and derivatives of active compounds in silico. Descriptive experimental research method was conducted in January using samples of kesambi plants that grow on the island of Madura. Histochemical analysis was carried out by preparing fresh leaves through lower leaf incisions with secondary metabolite detection reagents (CuSO4, FeCl3, Wagner, Sudan III, AlCl3 & FeCl3+NaCO3) then microscopic color changes were observed. In silico testing aims to determine the interaction of active compounds with ER? as a target for ER + breast cancer therapy through molecular docking. Supporting software used is KNApSAcK, Pubchem, Pass Online, PDB ID, PyRx, PyMol and Chimera 1.14. The results showed that through histochemical analysis six secondary metabolite compounds were identified, namely terpenoids, flavonoids, alkaloids, tannins, lipophils and phenols. While in silico analysis, the active compound is scopoletin which is derived from phenol, beta-sitosterol, betulin, betulinic acid, lupeol, lupeol acetate, schleicheol 1&2, schleicherastatin 1-7 which are derivatives of terpenoids. Based on the results of molecular docking, there are interactions of active compounds with 3ERT protein, the compounds that provide the most effective results as candidates for breast cancer drugs are lupeol acetate with a value of Root Mean Square Deviation (RMSD) lb 1,588 Å and ub 2,219 Å . Lupeol acetate compound is predicted to have activity as an Er? inhibitor against ER+ breast cancer. Keywords: Kesambi (Schleichera oleosa), histochemistry, molecular docking and ER?.