Rose myrtle fruit (Rhodomyrtus tomentosa, (W.Ait), Myrtaceae) is one of fruits widely found in Kalimantan. This fruit contains a bioactive compound that has a potential to be used as medicine. The aim of this study was to obtain optimal temperature and time of extraction in maintaining and protecting the bioactive compound in rose myrtle fruit extract by using water as solvent. This research applied the response surface method with central composite design for two factors, namely, X1 (temperature/°C) consisting of three levels: 70, 80, and 90°C and X2 (time/minute) which consisted of three levels of 60, 90, and 120 minutes. Research parameters included total phenol and antioxidant activity. Moreover, GC-MS was used for the characterization of the chemical compound component contained in rose myrtle fruit extract. Optimization of extraction condition resulted in an optimum temperature for extraction of 80.43°C and optimum time for extraction of 85 minutes with an optimum yield of total phenol of 73.77 mg/100 g fresh fruit and antioxidant activity of 1.0385 µg/ml with desirability of 0.892 or 89.2%.

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The purpose of this study was to determine the content of bioactive compounds, chemical content and antioxidant activity of karamunting fruit. The dietary fiber content of 100 grams of karamunting fruit is 83.37%, vitamin E (alpha-tocopherol) 10.25 mg/100 fresh fruit, lipids (2.96% of the RDI) with the predominant fat being linoleic acid (67.25% of total fatty acids), and sugar (6.25% of the RDI). TPC (Gallic Acid Equivalent)/total phenol 12.05 mg/100 g, total anthocyanins 3.63 mg/gram, total flavonoids 23.51 mg QE/gram, every 100 grams of karamunting fruit contains 4.6 grams of protein, and antioxidant (Inhibition Concentration 50) 1,028 (μg/ ml) is categorized as very strong, and 33 bioactive compounds. The karamunting plant that grows on Tarakan Island, North Kalimantan Province-Indonesia, which so far has no economic value and is underutilized, is proven to contain bioactive compounds that have very high antioxidant values, besides that they also contain high dietary fiber, essential fatty acids, and beneficial phenolic compounds. for health. In karamunting fruit, 33 bioactive compounds were also found, and 17 of them were new bioactive compounds that had not been discovered by previous researchers.