HighlightsCharacterization, antioxidant and immunomodulatory potential of exopolysaccharide (EPS) produced by wild and mutant Weissella confuse was evaluated.Wild Weissella confusa (WWCEPS) produced the highest EPS compare to the mutant strain.The EPS were heteropolysaccharides containing eight (8) monosaccharides in which galactose had the highest composition (34.60 mg/g and 33.47 mg/g EPS) in wild Weissella confusa EPS (WWCEPS) and mutant Weissella confusa EPS (MWCEPS).WWCEPS and MWCEPS had antioxidant capacity and WWCEPS had the highest DPPH capacity, total antioxidant activity, hydrogen peroxide and reducing power activity (71%, 1.88%, 86.7% and 1.85%).Wild and mutant Weissella confusa EPS exhibited immunomodulatory activity on the treated mice.