RNAi Double-stranded RNAs (dsRNAs) were made using gld-2 cDNAs (pJK830, exons 2-8 or pJK831, exons 16-18) as templates. Young adults were either injected with 2 mg ml 21 gld-2 dsRNA or soaked in 10 ml of 2 mg ml 21 gld-2 dsRNA for 12 h at 20 8C or mock-treated by injection with M9 buffer. Embryos were collected at defined intervals after treatment and processed together. Poly(A) polymerase assayProteins were in vitro translated using the TNT coupled transcription-translation system (Promega), and assayed using buffer conditions essentially as described 26 . For scintillation counting, poly(A) (Roche) was used as substrate. For gel assays, we used RNA oligo, C 35 A 10 (Dharmacon), a 45-nucleotide and supplemental 1 mM MgCl 2 . Products were analysed on 12% sequencing gels.

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Mps1 regulates its own turnover at kinetochores to ensure mitotic checkpoint silencing in metaphase.