P53 is important for cell cycle regulation, and its overexpression is seen in malignant tumors. We examined correlation between p53 expression and cell proliferation, and its role in the pathogenesis of keratinocyte skin tumors. We used biopsies from patients with squamous cell carcinoma, actinic keratosis and keratoacanthoma. We examined crosssections stained with HE and using anti-cytokeratin, antip53 and anti-Ki67 antibodies. Expression of p53 is found in 87, 85% of SCC, in 83. 3% of AK and 13. 4% KA. The high index of p53 expression was higher in SCC and AK compared to KA. We also observed a positive correlation between the expression of p53 and localization of the tumors. The largest proportion of subjects with AK and SCC has a high index of p53 expression on photoexposed region. We also observed that p53 expression correlates with age whereby in AK p53 expression increases with age. The high index of proliferation is most frequent in SCC and KA. Also at AK we found a strong correlation between a moderate proliferation index and tumor localization in photoexposed region. Between the proliferation index and p53 expression we observed a significant positive correlation only in SCC. Proliferation index and the expression of p53 are useful for the differentiation of precursor keratinocyte lesions and skin carcinoma. High p53 expression has been associated with the aging and significantly correlates with the exposure to UV radiation in SCC and AK. High expression of p53 in AK and SCC supports the importance of this oncoprotein in carcinogenesis of the skin.

Apstrakt Karcinom dojke je najčešća maligna neoplazmu u žena, čini oko 25% svih svih malignih bolesti i 15% svih smrtnih slučajeva, u ženskoj populaciji. Cilj našeg rada je ispitivanje učestalosti karcinoma dojke u pirotskom okrugu u periodu od 1998-2017 godine. Za ispitivanje smo koristili histopatološke izveštaje Službe za patologiju Opšte bolnice u Pirotu. Istraživanjem je obuhvaćeno 573 pacijenta operisana od karcinoma dojke u periodu od 1998-2017god. Analizirani su broj obolelih,vreme nastanka bolesti, starosno doba pacijenta u vreme dijagnoze, pol i histopatološki tip tumora. Za ispitivanje statističke značajnosti između grupa korišćen je Studentov t test. Karcinom dojke je verifikovan u 558 (97.38%) žena i u 15 muškaraca (2.62%). Prosečna starost žena sa karcinomom dojke u vreme dijagnoze je 62 god, a prosečna starost obolelih muškaraca je 64 godine Najveća učestalost karcinoma dojke zabeležena je u 2017 godini, pri čemu je zapažen statistički značajan porast broja obolelih od 2007 do 2017 godine. U radu se diskutuje o mogućim patogenetskim faktorima koji su uticali na signifikantan porast incidence karcinoma dojke u pirotskom okrugu. AbstractBreast cancer is the most frequent malignant neoplasm in women, accounting for about 25% of all the malignancies and 15% of all fatalities in female population. The purpose of our work is to examine the

SAŽETAKCilj. Cilj našeg rada je ispitivanje mikrovaskularne gustine (MVD) u core biopsijama karcinoma prostate (PC).Metoda. Na core biopsijama PC (n=25 ) i benigne hiperplazije prostate(BHP), served as controls (n=20) su, nakon rutinske obrade, primenjene klasična HE i imunohistohemijska ABC metoda sa CD105 antitelima. Svi ispitanici su imali nivo serumskog PSA >4.0 ng/mL. Srednja vrednost volumena prostate je 50.02, prosečan Gleason score je 7.7. MVD se računala brojanjem CD105 pozitivnih struktura u 5 "vrućih tačaka". Nakon kvantitativne analize, stereometrijski je izračunata MVD po mm 2 . .Za statističku obradu dobijenih rezultata korišćen je programski paket SPSS (verzija 19.0).Rezultati. Srednja vrednost CD 105 pozitivnih ćelija po mm 2 je značajno veća u PC nego kod BHP (676.6 vs 186.5). U PC postoji signifikantna povezanost MVD sa Gleason score (r=0.831) i nivoom PSA (r=0.343). Između PSA i volumena prostate postoji umerena pozitivna povezanost (r=0.565).Zaključak. Vrednost MVD indeksa moze biti značajan indikator progresije karcinoma prostate, tako da je moguće razlikovati pacijente kod kojih je ovaj rizik veći.Ključne reči: rak prostate, mikrovaskularna gustina, core biopsija, povećan rizik, progresija ABSTRACTObjective. Objective of our study was to investigate microvascular density (MVD) in core biopsies of prostate cancer (PC). On core biopsies of the prostatic carcinoma PC (n = 25) the benign prostatic hyperplasia (BPH) served as control (n = 20), and after routine processing, classical HE and immunohistochemical ABC method with CD105 antibodies, were applied.Method. All subjects had a serum PSA> 4.0 ng / mL. The mean value of prostate volume was 50.02, the average Gleason score was 7.7. MVD was achieved by counting the number of CD105-positive structures in the 5 "hot spots". After quantitative analysis, MVD was calculated stereometrically per mm2. For the statistical analysis of obtained results the software package SPSS (version 19.0) was used.Results. The mean value of CD 105-positive cells per mm2 was significantly higher in PC than in BPH (676.6 vs 186.5). There is a significant correlation of MVD with Gleason score (r = 0.831) and PSA (r = 0.343) in the PC. Between the PSA and prostate volume exists a moderate positive correlation (r = 0.565).Conclusion. Value of MVD index can be a significant indicator of the prostatic cancer progression degree in the core biopsy, thereby it is possible to distinguish patients with higher risk.

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