ABSTRACTThe Cry2Aa3 gene was introduced into asporogenicBacillus thuringiensis, and the synthesized protoxin killedBombyx moriandLymantria disparlarvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides killed the larvae of both insects.
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