PurposeThe purpose of this study was to quantify variations in the heart, pericardium, and left ventricular myocardium (LVM) caused by cardiac movement using the breath-hold technique.Patients and methodsIn this study, the electrocardiography-gated four-dimensional computed tomography (CT) images of 22 patients were analyzed, which were sorted into 20 phases (0–95%) according to the cardiac cycle. The heart, pericardium, and LVM were contoured on each phase of the CT images. The positions, volume, dice similarity coefficient (DSC) in reference to 0% phase, and morphological parameters (max 3D diameter, roundness, spherical disproportion, sphericity, and surface area) in different phases of the heart, pericardium, and LVM were analyzed, which were presented as mean ± standard deviation.ResultsThe mean values of displacements along the X, Y, and Z axes respectively were as follows: 1.2 mm, 0.6 mm, and 0.6 mm for the heart; 0.5 mm, 0.4 mm, and 0.8 mm for the pericardium; and 1.0 mm, 4.1 mm, and 1.9 mm for the LVM. The maximum variations in volume and DSC respectively were 16.49%±3.85% and 10.08%±2.14% for the heart, 12.62%±3.94% and 5.20%±1.54% for the pericardium, and 24.23%±11.35% and 184.33%±128.61% for the LVM. The differences in the morphological parameters between the maximum and minimum DSC phases for the heart and pericardium were not significantly different (p>0.05) but were significantly different for the LVM (p<0.05).ConclusionThe volumetric and morphological variations of the heart were similar to those of pericardium, and all were significantly smaller than those of the LVM. This inconsistency in the volumetric and morphological variations between the LVM and the heart and pericardium indicates that special protection of the LVM should be considered.