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The structures of the A^-linked oligosaccharides of momordin-a, which is a ribosome-inactivating protein from the seeds of Momovdicacharantia, were analyzed. First, the TV-linked oligosaccharides of this glycoprotein were liberated by hydrazinolysis. After N-acetylation, the reducing ends of the oligosaccharides were coupled with 2-aminopyridine and the pyridylamino (PA-) derivatives were purified by gel filtration and high performance liquid chromatography (HPLC) on an ODS-silica column. Three kinds of oligosaccharide fractions were separated by HPLC. The structure of each oligosaccharide isolated was analyzed by a combination of sugar component analysis, exoglycosidase digestion, another kind of HPLCusing an amide-silica column, and 500-MHz *H NMRspectroscopy. Thestructures of two mainoligosaccharides wereestablished to be:Xyl l /?2Man/J4GlcNAc/*4GlcNAc Momordin-a, which inactivates 60S ribo-

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