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SummaryThe emission of N 2 and N 2 O from intact transgenic tobacco (clone 271) expressing antisense nitrite reductase (NiR) mRNA, and wild-type plants grown aseptically, on NO 3 ± , NO 2 ± or NH 4 + -containing medium was investigated. 15 N contents of gas sampled from gassealed pots, in which the plants were grown on 15 Ncontaining medium, were analyzed by gas chromatography and mass spectrometry (GC±MS

Sixty one green tea samples with price ranging from 560 to 12,000 yen/kg were collected and the contents of total nitrogen and individual free amino acids were determined. There was no difference in their glutamic acid contents between samples, however theanine, glutamine and arginine contents varied significantly depending on the price of green tea. Among the higher price teas, glutamine was more than glutamic acid, while in lower price teas it was the reverse.Among low and middle price teas, the price corelated to total nitrogen content, while teas with high price, no corelation was observed. Therefore, it seemed to be impossible to estimate the quality of high price tea from the total nitrogen content. The higher correlation was observed between

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