BackgroundAlthough a 3-arm DOTA construct, which has three carboxylic acids, h has been applied for conjugation to many peptides, we investigated if a 4-arm DOTA construct conjugated to peptides improves chemical properties for melanoma imaging of the melanocortin 1 receptor compared to 3-arm DOTA-conjugated peptides.MethodsSpecific activities, radiolabeling efficiencies, and partition coefficients were evaluated using 111In-labeled 3-arm and 4-arm DOTA-α-melanocyte-stimulating hormone (MSH). For assessment of MC1-R affinity and accumulation in tumor cells in vitro, B16-F1 melanoma and/or 4T1 breast cancer cells were incubated with 111In-labeled 3-arm and 4-arm DOTA-α-MSH with and without α-MSH as a substrate. The stability was evaluated using mouse liver homogenates and plasma. Biological distribution and whole-body single photon emission computed tomography imaging of 111In-labeled 3-arm and 4-arm DOTA-α-MSH were obtained using B16-F1 melanoma-bearing mice.ResultsSpecific activities and radiolabeling efficiencies of both radiotracers were about 1.2 MBq/nM and 90–95%, respectively. The partition coefficients were −0.28 ± 0.03 for 111In-labeled 3-arm DOTA-α-MSH and −0.13 ± 0.04 for 111In-labeled 4-arm DOTA-α-MSH. Although accumulation was significantly inhibited by α-MSH in B16-F1 cells, the inhibition rate of 111In-labeled 4-arm DOTA-α-MSH was lower than that of 111In-labeled 3-arm DOTA-α-MSH. 111In-labeled 4-arm DOTA-α-MSH was taken up early into B16-F1 cells and showed higher accumulation than 111In-labeled 3-arm DOTA-α-MSH after 10 min of incubation. Although these stabilities were relatively high, the stability of 111In-labeled 4-arm DOTA-α-MSH was higher than that of 111In-labeled 3-arm DOTA-α-MSH. Regarding biological distribution, 111In-labeled 4-arm DOTA-α-MSH showed significantly lower average renal accumulation (1.38-fold) and significantly higher average melanoma accumulation (1.32-fold) than 111In-labeled 3-arm DOTA-α-MSH at all acquisition times. 111In-labeled 4-arm DOTA-α-MSH showed significantly higher melanoma-to-kidney, melanoma-to-blood, and melanoma-to-muscle ratios than 111In-labeled 3-arm DOTA-α-MSH.ConclusionsThe 4-arm DOTA construct has better chemical properties for peptide radiotracers than the 3-arm DOTA construct.

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Proposal of a drainage stack system with a waste disposer for application to real super high-rise commercial buildings and the verification of the drainage performance thereof (Report 1)

This report describes a commercial disposer drainage stack system installed to an approx. 100 m tall super high-rise experimental drainage tower, and the examination of how large amounts of waste drained from the kitchen of the tower (kitchen waste) affects the drainage performance of the stack and the carrying performance of the house drain. The report also aims to acquire, on the basis of the experiment results, useful technical knowledge necessary for the actual planning and design of a disposer drainage stack system which is used specifically for real super high-rise buildings for the business use. Masayuki Otsuka Hideaki Hoshina Toshitaka KatoThis report describes a commercial disposer drainage stack system installed to an approx. 100 m tall super high-rise experimental drainage tower, and the examination of how large amounts of waste drained from the kitchen of the tower (kitchen waste) affects the drainage performance of the stack and the carrying performance of the house drain. The report also aims to acquire, on the basis of the experiment results, useful technical knowledge necessary for the actual planning and design of a disposer drainage stack system which is used specifically for real super high-rise buildings for the business use.

This research aims to assess the greenhouse gas (GHG) emissions reductions due to the use of biogas technology in Quang Tri Province. With a total of over 354,000 cattle in Quang Tri Province, Vietnam, waste from livestock becomes large. The GHG emitted from the livestock industry is not small, affecting the environment. Currently, there is little concern or documentation about the reduction of GHG emissions in small farms using biogas digesters in central Vietnam. This province has applied technological solutions, typically biogas digesters, but the amount of biogas production is not calculated accurately. Our survey was conducted in Vinh Linh District and Cam Lo District in March 2019 and involved 50 farms equipped with biogas digesters and 20 farms without it. The respondents were selected based on the information provided by local authorities, satisfying two conditions: livestock households and biogas users. The former group was asked 25 questions and the latter was asked 10 questions needed to calculate GHG emissions such as the number of animals and petroleum gas/ firewood consumption. This study uses formulas described in the 2006 guideline issued by IPCC to estimate reduced GHG emissions. The results showed that the average biogas production is 5.52 m³.household-1.day-1. Only 2% of the farms made the best use of the biogas digester. The surveyed households have not really used the most optimal amount of biogas production. In this scenario, this study recommends some solutions for solving the problem. In addition, the average annual emissions before having a biogas digester are estimated to be 20.53 tons CO2e/household/year. After using the biogas, the GHG emissions are reduced to 4.52 tCO2e.household-1.day-1. Thus, the replacement of daily cooking energies with biogas helps reduce 16.01 tCO2e of greenhouse gas for each farm per year.