The volume, maximum horizontal area, maximum length, maximum width and depth of the seminal vesicles were measured by transrectal ultrasonography in 76 subjects without seminal vesicle disorders, divided into 7 groups at 10-year intervals. The average of each parameter in each group was presented. The volume and maximum horizontal area began to decrease after the age of 60 years. No statistically significant difference among subject ages was observed for the other 3 parameters. A correlation was observed between the volume and maximum horizontal area of the seminal vesicles. Our study confirmed that maximum horizontal area of the seminal vesicles may be substituted for the volume.
A continuous sampling model of the fluid excreted from the unilateral seminal vesicle was devised in the rat. Each 12 hr change in the volume was measured in 14 sexually mature male rats by use of tubing. If we assume the excretion of a small volume (less than 4 pl) as the resting level, 3 of 14 rats showed only the resting level. The excretion of a large volume was sometimes observed in the 11 other rats both in daytime and nighttime. This model would be promising for investigations of the seminal vesicles from new aspects. seminal vesicles ; continuous sampling model ; qualitative analysis ; cannula swivelWe have devised a new continuous sampling model of the seminal vesicle fluid in the rat. The method will be reported briefly in this papaer.
MATERIALS AND METHODSFourteen male rats (14-week-old, Wistar Strain) weighing between 400 and 420 g were used. Under pentobarbital anesthesia, the seminal vesicles were exposed through an abdominal median incision and the unilateral seminal vesicle with the coagulating gland was ligated close to the posterior urethra so as to exclude the arteries and veins. The contralateral seminal vesicle was left intact.An INTRAMEDIC PE 50 polyethylene tube (0.58 mm in inner diameter) filled with heparinized saline was then inserted into the ligated seminal vesicle. The seminal vesicle, thus, was replaced in the abdomen and the incision was closed. The tube was passed through a subcutaneous tunnel and exteriolized at the vertex of the head. The tube was connected to a cannula swivel through a protective coil. The second tube was attached to the other end of the cannula swivel. The other end of the second tube was placed at the same level of the seminal vesicle to keep the static pressure (Fig. 1). After the operation, the rats were kept for about one week without any restriction in the cage. Each 12 hr change in the volume was recorded by marking the end of the saline in the tube at 9 a.m. and 9 p.m.
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