No abstract

The objective of selecting strains of Bacillus sp. has high activity in carbon metabolism for peat treatment, firstly focusing on the ability to degrade lignin, cellulose, phosphorus and protein. From the gene pool, by surveying the microbial strains capable of degrading carbon-rich substrates including lignin, cellulose, insoluble phosphate, proteolytic, we have identified two Bacillus sp. strains contain Bacillus polyfermenticus and Bacillus subtilis strains have strong ability to metabolize carbon-rich substrates and decompose phosphorus in peat. The Bacillus polyfermenticus strain had cellulose, lignin, organophosphorus and starch-degrading ring diameters of 41, 40, 23 and 38 mm, respectively. The Bacillus subtilis strain had cellulose, lignin, organophosphorus and starch-degrading ring diameters of 40, 41, 24 and 29 mm, respectively. In addition, the content of humic acids formed after 48 hours of fermentation increased 2.5 times compared to the original without the addition of Bacillus polyfermenticus strain and increased 2.8 times compared to the original without the addition of Bacillus subtilis strain. Therefore, two strains of Bacillus sp. selected with high efficiency in producing humic acid products. At the same time, the selected microbial strains have a high biosafety level (level of 1, the safest level in the biosafety scale).