Sustainable insect supply is a routine activity in research of pest control, experimental insecticide bioassay, entomophatogen, parasitoid, and nature of competitor. Armyworm (Spodoptera litura Fabr.) is one of the alternative test insect. A mass rearing method is required to provide high demands of S. litura. A life table of insect is regularly used to study several biological parameters including the net reproduction rate of population (Ro), the mean length of life period in one generation (T), reproduction potential value of one generation from a population (rm), and the potential of a population to multiply in one generation (ë). The aim of this study were to asses the development time and reproduction potential of S. litura which different of artificial diets. Results showed that S. litura on artificial diet in recipe of Singh and Moore is T = 29,90, R = 2692,33, r = 0,26, ë = 1,29, o m whereas artificial diet in recipe of Waldbauer et al. is 32,29, R = 891,99, r = 0,21, ë = 1,23. o m

Nutrition indices and survivorship of Spodoptera Litura F. larvae is fed nutrition including jengkol bark extract (Pithecellobium Lobatum Benth).  The effect of  P. lobatum barks extract on nutrition indices and survivorship S. litura larvae has been investigated. Nutrition indices were observed by giving fifth-instar larvae one of the three diets, every group of diet was added various concentration of barks extract, containing either 1.10; 2.20; or 4.40%. Survivorship were observed by giving first-instar larvae one of the three diets, every group of diet was added various concentration of barks extract, containing either 0.6; 1.2; or 2.4%. The result showed that, the Relative Consumption Rate (RCR),  Relative Growth Rate (RGR), Efficiencies of food use (ECD and ECI) value of larvae treated were significantly lower in the case of treated larvae than that of the controls, Approximate Digestibility (AD) value of larvae treated were significantly higher in the case of treated larvae than that of the controls and decrease of survivorship tested larvae.

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Antifeeding activity of soursop leaf extract    (Annona muricata L.) and its effect on nutrition indices and the microscopic structure of peritrophic membrane of the fifth-instar larvae of Spodoptera litura F. were studied.  Antifeeding test was conducted by choice method. Discs of caisin leaf were dipped into the ether fraction of soursop leaf extract with concentrations of 0; 0.63; 2.50; and 10.00%. Nutrition indices and peritrophic membrane structure were observed by giving fifth-instar larvae  one of the five diets, every group of diet was added to various concentrations of  the ether fraction of soursop leaf extract, containing either 0; 0.63; 1.25; 2.50; and 5.00%. The result showed that  soursop leaf extract had antifeeding activity at tested concentration 2.50%. Relative consumption rate (RCR), relative growth rate (RGR), and  efficiency of conversion of  ingested food  (ECI) were significantly lower in the case of treated larvae than that of the controls. However the efficiency of conversion of digested food  (ECD), approximate digestibility (AD), and peritrophic membrane structure treated with  soursop leaf extract were not significantly affected as compared to those in  controls.

DBD dan chikungunya merupakan salah satu penyakit yang masih menjadi masalah di Indonesia. Kecamatan Cilongok merupakan salah kecamatan endemis DBD dan pernah mengalami KLB chikungunya. Deteksi virus pada nyamuk sebelum menginfeksi manusia penting sebagai peringatan dini dalam upaya pencegahan wabah di daerah endemis. Tujuan penelitian ini adalah mengetahui infeksi virus Dengue dan Chikungunya pada nyamuk Aedes spp. yang ditangkap. Penelitian ini dilakukan di empat desa di Kecamatan Cilongok yang meliputi Desa Cilongok, Pernasidi, Kalisari, dan Jatisaba, pengambilan sampel dilakukan secara purposive. Deteksi virus Dengue dan Chikungunya pada nyamuk dilakukan menggunakan metode Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR). Hasil positifitas virus dianalisis secara deskriptif untuk menggambarkan potensi transmisi virus. Hasil penelitian menunjukkan bahwa nyamuk Aedes spp. yang tertangkap tidak mengandung virus Dengue dan Chikungunya.