The increase in total Kjeldahl nitrogen (TKN) concentrations is correlated with increases in cyanobacterial bloom biomass. Standard methods for the measurement of TKN are tedious, costly, time-consuming and involve the use of hazardous catalysts, such as mercury, high temperatures and significant amounts of toxic acids and bases. Since TKN plays a pivotal role in influencing algal blooms, there is an urgent need to develop simpler, safer and more accurate methods for the determination of TKN. The simplified TKN method (s-TKN™) developed by Hach® offers several advantages over the traditional TKN method, including eliminating the use of mercury, requiring low sample and reagent volumes and being cost-efficient and user-friendly. This communication presents preliminary results comparing the efficacy of s-TKN™ and the standard method, using commonly used primary standards and waste, estuarine and lake water matrices. For all primary standards analyzed, the s-TKN™ method exhibited good accuracy across a wide range of concentrations. The repeatability for the glycine–para-toluene sulfonic acid (Gly-PTSA) standard using the s-TKN™ method was 4.1% at the highest concentrations analyzed, with overall repeatability across concentrations comparable to the standard EPA method. For wastewater, estuarine and lake matrices, a good correlation (r2 = 0.9917) between the two methods and no statistical difference in the values (p > 0.05) were obtained between two methods. Preliminary studies indicate that the s-TKN™ method has the potential to reduce the expenditure associated with the cost of analysis and has the potential to be a safer and cheaper alternative, while providing comparable analytical results to the standard method.