Introduction: Gender-based preferences are common in dental practice and maybe even more prevalent in academic dentistry. A large number of females have been entering the practice of general dentistry for the past two decades. The present study was done to assess dental students' perception of gender preferences in dentistry using a questionnaire in Saudi Arabia. Materials and Methods: A survey was conducted using a pretested questionnaire among dental interns pursuing their internship at different dental schools in Saudi Arabia. The questionnaires were distributed to 482 interns who were willing to participate in the survey. All the responses were collected, tabulated, and analyzed accordingly using appropriate statistical methods. Results: Four hundred and eighty-two dental interns (180 males and 302 females) responded to the survey. Most female interns (68%) prefer female patients for their treatments to the opposite gender. Seventy-five percent of the male interns felt more capable than females to handle stress and workload associated with branches such as prosthodontics, surgery, and implantology. 52.1% felt that female students fared well in academics. Conclusion: The present study shows that more females are taking up this profession in the current situation. It also shows that females are as competitive as males in dentistry and that most of the dental interns want to take up postgraduation after completion of the course. Both males and females are given equal working space and respect in this field of dentistry.