A simple, precise and accurate method to determine ampicillin and dicloxacillin in a pharmaceutical preparation capsule dosage form were developed and validated using liquid chromatography (LC). The LC separation was achieved on an ACE 150 mm x 4.6 mm, 5 µ in the isocratic mode using buffer: methanol (40: 60, v/v), as the mobile phase at a flow rate of 1.0 mL/min. The methods were performed at 220 nm. In LC method, quantification was achieved with PDA detection over the concentration range of 50 to150 µg/mL. The methods were validated and the results were compared statistically. They were found to be simple, accurate, precise and specific. The methods were successfully applied for the determination of ampicillin and dicloxacillin in a pharmaceutical preparation capsule dosage form without any interference from common excipients. The proposed method was validated in terms of precision, robustness, recovery, LOD and LOQ. All the validation parameters were within the acceptance range.

A simple and accurate method to determine tadalafil (TAD) in pure powder and tablet dosage form was developed and validated using HPLC. The separation was achieved on an Xterra RP18 column (150 4.6 mm id, 3.5 m) in the isocratic mode using bufferacetonitrile (70 + 30, v/v), adjusted to pH 7.00 0.05 with triethylamine as the mobile phase at a flow rate of 1.0 mL/min. The photodiode array detector was set at 225 nm. Quantification was achieved over the concentration range of 50.7152.10 g/mL with mean recovery of 100.26 0.75. The method was validated and found to be simple, accurate, precise, and specific. The method was successfully applied for the determination of TAD in pure powder and tablet dosage form without interference from common excipients or degradation products.

A simple and accurate methods to determine donepezil, in tablet dosage form, were developed and validated using liquid chromatography (LC). The LC separation was achieved on a Inertsil C8-3, 25 cm x 4.6-mm, 5 µ in the isocratic mode using buffer : methanol : triethylamine (550:450:5)v/v, adjusted to pH 2.50±0.05 with orthophosphoric acid, as the mobile phase at a flow rate of 1.0 mL/min. The methods were performed at 271 nm. In LC method, quantification was achieved with PDA detection over the concentration range of 20-60 µg/mL with mean recovery of 100.18±0.75%. The method was validated by determining its sensitivity, accuracy and precision. The proposed method is simple, fast, accurate and precise and hence can be applied for routine quality control of donepezil HCl in bulk and tablet dosage form

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