The <em>Aedes aegypti</em> mosquito lays its eggs in calm and clear water. People often collect water for daily needs which is placed outside or inside the house, water comes from tap water, rainwater, and well water, provide a good place for <em>Aedes aegypti </em>to breed. The <em>Aedes aegypti</em> as a vector of the Dengue Virus and others. The prevalence of Dengue Fever in Pekalongan City in 2017, as many as 44 cases of DHF in the Medono Community Health Center area. The purpose of this study was to determine the hatchability of <em>Aedes aegypti</em> eggs in the well water, tap water, and rainwater in Medono Village, Pekalongan City. The research was carried out at the Research and Development Center for Disease Vector and Reservoir Research Laboratory (B2P2VRP) Salatiga. The sample consisted of 540 eggs of <em>Aedes aegypti. </em>This research is descriptive, which makes observations and examinations in the laboratory by inserting <em>Aedes aegypti</em> eggs into three types of water, with nine replications for each type of water, each glass filled with 20 mosquito eggs, and observed for 24 hours. Research also measures water temperature and pH. The results show the average number of mosquito eggs hatched in well water as many as 6.89 (34.45%), rainwater 4.44 (22.2%), and tap water 2.67 (13.35%). The pH of the well water is 7.56, rainwater is 7, and tap water is 6. The temperature of the three types of water is the same, which is 25^O C. The conclusion of this research is the hatchability of <em>Aedes aegypti</em> eggs in well water as many as 6.89 (34.45%), rainwater 4.44 (22.2%) and tap water 2.67 (13.35%). Tap water with a lower pH has a lower hatchability of <em>Aedes aegypti</em> eggs.

The Blood Smear is a slide whose one of the surfaces is coated using a thin layer of blood and stained with painting of giemsa or wright. Before painting, the preparation is fixated with methanol (methyl alcohol). Prolonged fixation should be avoided because methanol affects subsequent painting and causes some inhibition of intracellular enzymes. Methanol that is left too long in the open air will evaporate (decrease in concentration) and contain water so that it will affect erythrocyte morphology. This study aims to find out the effect of variations in sadt fixation time on giemsa painting on the morphology of red blood cells. This type of research is an experiment.  The sample numbered 30 in the form of peripheral blood smear preparations divided by 3 based on variations in fixation time of 3 minutes, 5 minutes and 10 minutes. The results of the Chi-Square statistical test obtained a signification value of 0.00 < 0.05 means that there is a significant difference in time variation in the morphological quality of cells. And the fixation time is best with a fixation time of 3 minutes, at a fixation of 5 minutes still found some crenation cells (cell grinding) with moderate criteria (<3) while fixation with a time of 10 minutes is found a lot of crenation cells (>3).

Sifilis adalah penyakit infeksi disebabkan oleh Treponema pallidum merupakan penyakit kronis dan bersifat sistemik. Penyakit sifilis dapat ditularkan dari ibu yang menderita sifilis ke janinnya melalui plasenta pada stadium akhir kehamilan. Untuk mengetahui seseorang terinfeksi sifilis diantaranya dengan pemeriksaan imunoserologi yaitu pemeriksaan VDRL (Veneral Disease Research Laboratory) dengan metode slide flokulasi adalah tes yang dilakukan untuk memeriksa munculnya antibodi terhadap bakteri Treponema pallidum, bakteri yang menyebabkan penyakit menular seksual sifilis. Tujuan Penelitian ini adalah untuk mengetahui gambaran hasil pemeriksaan sifilis pada ibu hamil di Puskesmas Tirto II Kabupaten Pekalongan. Jenis penelitian yang digunakan adalah deskriptif. Jumlah sampel sebanyak 33 sampel diambil secara accidental sampling dengan pemeriksaan VDRL metode slide menggunakan prinsip flokulasi. Hasil penelitian ini menunjukkan bahwa usia ibu hamil terbanyak ditemukan pada usia 20-29 tahun yaitu 18 orang (55%), jumlah anak terbanyak ditemukan pada ibu hamil yang baru mempunyai 1 anak yaitu sebanyak 11 orang (33%), usia kehamilan 9 bulan paling banyak ditemukan yaitu 12 orang (36%), pernah menderita penyakit menular seksual hanya ditemukan 2 orang (6%), tidak pernah menderita penyakit menular seksual lebih banyak ditemukan yaitu 31 orang (94%), mayoritas responden tidak menunjukkan gejala seperti benjolan dan luka yaitu sebanyak 31 orang (94%), namun menunjukkan gejala ruam merah sebanyak 2 orang (6%). Dari 33 sampel ibu hamil didapatkan hasil bahwa 2 sampel reaktif (+) dengan persentase (6%) dan 31 sampel non reaktif (-) dengan persentase (94%). Dari 33 sampel serum ibu hamil di Puskesmas Tirto II Kabupaten Pekalongan didapatkan hasil 2 sampel ibu hamil reaktif (+) sifilis