Work-life balance is one of the prevailing issues in many organizations. Inability of workers to balance work and family could lead to increased rates of absenteeism, stress, employee turnover, job dissatisfaction and poor work performance. It is therefore essential for employees to maintain stability between work and their private lives. This research examined the effect of work-life balance on employee performance of selected deposit money banks in Lagos State, Nigeria. Survey research design was adopted. The target population was 54,028 employees, which consisted of all employees of the six selected Deposit Money Banks in Lagos State, Nigeria. A sample size comprising 520 employees were selected using random sampling technique. Primary data were collected using a structured questionnaire. Data were analysed using descriptive statistics and simple regression analysis. The findings revealed that job stress significantly affects quality of work of employees (F= 33.500, p< 0.05); and long working hours significantly affects motivation of employees in the selected deposit money banks in Lagos State (F= 8.716, p< 0.05). However, the study reveals that role overload has no significant effect on job satisfaction of employees in selected deposit money banks in Lagos State (F= 0.011, p> 0.05). This study concludes that work-life balance has significant effect on employee performance in the selected deposit money banks in Lagos State, Nigeria.