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<italic>Lactobacillus casei</italic> group is commonly used as probiotics. Various factors affect their viability in gastrointestinal tract. Twenty two strains of <italic>Lactobacillus casei</italic> group, were assessed for acid tolerance attribute. ASCC 292 showed the highest viability at pH 1.5. Fifteen remaining strains have shown no activity after 1 h. At pH 2.0, ASCC 1002 has shown lowest viability after 2 h of incubation. At pH 2.5, all twenty two strains decreased their viable count after 3 h of incubation. Greater viable count of <italic>Lactobacillus</italic> strains were observed at pH 3.0. This study suggests the importance to identify all the useful <italic>Lactobacillus</italic> strains in order to study further for their therapeutic benefits.

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