The present work focus to evaluate the physicochemical and preliminary phytochemical studies on the seeds of family Caricaceae. The plants resources which are used in pharmaceutical formulation standardization was carried out on the basis of organoleptic properties, physical characteristics, and physico-chemical properties. Different Physicochemical parameters ash values, extractive values, loss on drying, foreign matter, fluorescence analysis, and pH were evaluated. Macroscopical characteristics and Physicochemical parameters like total ash, acid insoluble ash and water soluble ash were found to be 83.7%, 71.7% and 61.5% w/w respectively. Hexane, ethyl acetate, ethanol and water soluble extractive values (hot)were 7.6%, 11.6%, 27.4%, 37.5%w/w respectively. The pH of 1% and 10% aqueous solution was found to be 3.57 and 3.78 respectively. Preliminary phytochemical screening showed the presence of Tannins, Proteins and amino acids, Glycosides, Phenolic compounds, Carbohydrates, Saponins, Alkaloids and Flavonoids. Thin layer chromatographic studies also had been done on ethanolic and hexane extracts. HPTLC fingerprinting is a valuable method for the quantitative determination of phytochemicals present in plant extract. These studies aim to investigating referential information for correct identification and standardization of this plant secondary metabolites.
The present work focus to evaluate the physicochemical and preliminary phytochemical studies on the leaves of Prosopis cineraria (L.) Druce (family: Fabaceae). The plants resources which are used in pharmaceutical formulation standardization was evaluated on the basis of various organoleptic properties, physical characteristics, and physico-chemical properties. Different Physicochemical parameters ash values, extractive values, loss on drying, foreign matter, fluorescence analysis, pH, TLC and HPLC were evaluated. Macroscopically seeds the total ash, acid insoluble ash and water soluble ash were found to be 7.06%, 3.14%, and 69.3% w/w respectively. Hexane, ethyl acetate, ethanol and water soluble extractive values (hot)were 4.865 %, 19.092 %, 12.19%, 24.82%w/w respectively. The pH of 1% and 10% aqueous solution was found to be 6.4 and 6.8 respectively. Primary phytochemical screening indicated the existence of sterols, tannins, flavonoids, amino acids, glycosides, phenolic compounds, carbohydrates, saponins and alkaloids. Thin layer chromatographic studies also had been done on ethanolic and hexane extracts. HPTLC fingerprinting is a valuable method for the quantitative determination of phytochemicals present herbal medicine. HPTLC fingerprinting Showing the presence of Gallic acid in ethonolic extract.Rf value calculated and repoted.in plant organoleptic and physico-chemical properties changed due to geographical diversity and time of collection of sample. The present work therefore, attempts to report necessary pharmacognostical and standardization parameters the leaves of Prosopis cineraria (L.) Druce (family: Fabaceae) which will help to identify and development of the drug.
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