Background: Ulcerative colitis (UC) is an inflammatory bowel disorder affecting the colonic mucosa, characterized by intense inflammation and mucosal damage. The present study aimed to evaluate the protective effect of the Trigonellafoenum-graecum L. (TFG) seeds in acetic acid-induced UC in rats. Materials and Methods: Male rats (n=30) were distributed into 5 groups as normal control, UC, standard, and two test groups. Colitis was induced by acetic acid in all the groups except the normal control group. Normal control and UC group received distilled water, the standard group was administered sulfasalazine at 100mg/kg body weight (bw), and test groups, TFG-I, and TFG-II received TFG seed extract at 500 and 1000 mg/kg bw, respectively. The duration of treatment was 7 days, and colitis was induced on day 8. Animals were sacrificed on day 9 and colonic tissue was dissected and collected for biochemical, molecular, and histological analysis. Results: The disease activity index score in standard, TFG-I, and TFG-II (3.33±0.21, 2.66±0.21, and 3.50±0.22) was significantly lesser (P<0.05) than scores in the UC group (4±0.01). The macroscopic score indicating the intensity of mucosal inflammation was significantly decreased (P≤ 0.01) to4.0±0.25, 3.16±0.30, and 3.83±0.40 in standard, TFG-I, and TFG-II groups, respectively compared to the UC group (4.66±0.21). Similarly, there was a significant reduction (P≤0.05) in histological scores of the standard, TFG-I, TFG-II (3.5±0.34, 1.25±0.34, 3.25±0.34) groups compared to the UC group (4.75±0.34). Biochemical assessment in the standard and test groups showed significant increase (P<0.05) in total protein, reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) levels whereas significant reduction (P<0.01) in malondialdehyde (MDA) levels compared to UC group. The pro-inflammatory cytokine TNF-α levels were significantly decreased (P<0.01) in standard, TFG-I, and TFG-II (-1.75±0.007, -0.27±0.17 and -0.51±0.002) when compared to the UC group (0.20±0.02). Conclusion: The study demonstrates the ability of TFG seeds in reducing the inflammatory and oxidative stress induced mucosal damage in acetic acid-induced UC in rats.

Ulcerative colitis is an inflammatory disorder affecting colonic mucosa, characterized by intense inflammation and mucosal damage. The currently available medical treatment is not completely safe and effective in alleviating the disease manifestations and its complications. The present study aimed to evaluate the protective effect of herbal product Trigonella foenum-graecum L. (TFG) seeds in acetic acid induced Ulcerative colitis (UC) in rats. The TFG extract was administered orally at two doses of 500 and 1000 mg/kg body weight. Compared to UC group, the TFG treated group showed significantly reduced severity of the disease as indicated by decreased Disease Activity Index (DAI) and decreased intensity of mucosal inflammation as indicated by macroscopic and histological scoring. Furthermore, biochemical assessment showed increased total protein, reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and decreased malonaldehyde (MDA) and pro-inflammatory cytokine TNF-α levels in TFG treated group compared to the UC group. The study demonstrates the beneficial effect of TFG in UC by its natural anti-inflammatory and antioxidant properties.