Diesel oil is considered one of the important products of crude oil which constitute a vital source of pollution in the environment. Biodegradation of complex hydrocarbon requires cooperation of more than a single species. This is true in case of pollutants made up of crude oil or petroleum. Soil contains varieties of microorganism that can be established in any natural environment. The bacterial isolates were characterized biochemically by indole test, methyl red test, citrate utilization Test, voges proskauer test, starch hydrolysis, catalase production test, nitrate reduction test, antagonism test, oligodynamic susceptibility and antibiotic sensitivity tests.

The study basically focuses on hydrocarbon contamination that is caused because of bacteria present in the soil. Transport Nagar Area in Jaipur is mostly covered with garages and hence surrounded by crude oil, petrol, diesel and other fractions of crude oil. Soil and ground water are polluted with diesel fuel from leaking underground storage tanks which happens as a result of accidental spills and leakage from pipelines which causes harm to the both flora and fauna. In the study conducted the treatment samples were observed with significant difference with the control. The microbial count was maintained in the range of 3.86*104- THBP per gram soil and 1.87*103- PDBP per gram soil. The highest bacterial growth was showed after 60 days with 40% NPK because of the presence of an inorganic nutrition, bacterial activity and as a result petroleum degradation was maximum.Int J Appl Sci Biotechnol, Vol 4(2): 233-235