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Hydrogen peroxide is an agent that triggers oxidative stress. Glucose, which is a source of carbon and energy has a regulatory role in many metabolic processes such as growth rate, fermentation capacity and stress response. Schizosaccharomyces pombe has eight hexose transporters with a different affinity for glucose and/or related monosaccharides. In S. pombe, Ght5 is a glucose transporter with high-affinity. We aimed to investigate the effects of H 2 O 2 -induced oxidative stress on hexose transporters using glucose repression-resistant mutant strains (ird5 and ird11) of S. pombe. We analyzed the percentage of glucose consumption in S. pombe wild-type and mutant cells under stressed and non-stressed conditions. Then we compared the expression levels of the genes encoding hexose transporters under the same conditions. We confirmed that the glucose consumption efficiencies of the mutants were slower than the wild-type as in earlier study under non-stressed condition. The percentage of glucose consumption reduced by approximately two-fold in ird11 and wild-type, but not change in ird5, under a stressed condition. There is no difference between cells shape and size of S. pombe strains under stressed and non-stressed conditions. Under stress-induced condition, the expression levels of ght3, ght4 genes in ird11 and wild-type, and ght4, ght6 genes in ird5 decreased, but that of ght5 gene remarkably increased in only wild-type.We suggested that oxidative stress caused by H 2 O 2 leads to upregulation of the ght5 gene in S. pombe.

We generated highly chloroquine (CQ)-resistant (ResCQ) Plasmodium yoelii parasites by stepwise exposure to increasing concentrations of CQ and CQ-sensitive parasites (SenCQ) by parallel mock treatments. No mutations in genes that are associated with drug resistance were detected in ResCQ clones.