Aim:The purpose of the study was to analyze cytotoxicity caused by dental adhesives on human gingival fibroblast (HGF) cell lines, compare the response of cultured HGFs to substances leached from a conventional total etch and self-etching adhesive system along with their individual components. Methodology: Healthy gingival tissue biopsies (explants) were used to obtain isolates of HGFs, which were cultivated in flasks till subconfluent monolayers obtained. Serial dilutions for each compound tested were made and elutes at all concentrations tested for were obtained at 48 h for each material which were then added at different concentrations to the HGF cultures, and cytotoxicity was analyzed by 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: Within the limitations of the in-vitro study, we conclude: All of the materials tested showed moderately or slightly cytotoxicity at the lower serial dilutions except bisphenol alpha and One Coat-7.0, which proved to be strongly cytotoxic among almost all the dilutions. The lowest cytotoxicity was obtained from Clearfil SE Bond at 0.3125 mg/mL and the highest from One Coat-7.0 at 5.9 mg/mL, among the three dentin bonding systems tested for cytotoxicity. Conclusions: Indicated that SE B had the lowest mean % of cell viability value and OC had the highest mean % of cell viability.

Background: Over the last decade, a lot of anti-Bisphenol A (BPA) groups have emerged explaining the ill effects of its derivatives commonly used in dental products. Since these materials are used on a daily basis in our clinical practice, we must ensure their safety to all who are in contact with them. This is the primary motive of our study. Aim: The purpose of the study was to analyze cell death and cytotoxicity caused by a dental composite on lymphocytes, comparing the response of cultured lymphocytes to substances leached from a nanohybrid restorative resin at different time intervals. Methodology: The effect of composite on lymphocytes was studied in two groups, one at 24 h and the other at 72 h. Additional control groups were tested also for. On culturing the test materials with the cells, they were subjected to the ethidium bromide and acridine orange dyes to check vitality. Results: Within the limitations of this in-vitro study, it was concluded that no significant apoptosis was detected in the control group. Early-stage apoptotic cells were detected in the first experimental group of 1 day/24 h. Increasing concentrations and treatment lengths showed a decrease in the number of early-stage apoptotic cells in the 3 day group. Conclusions:The material tested at all intervals showed very less toxicity confirming low cell death. Clinical significance: In light of recent awareness related to all materials with BPA and its derivatives, we conducted this study with a commonly used restorative material.