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Practical and effective methods for the detoxification of aflatoxins in contaminated foods and feedstuffs are highly needed to reduce deleterious effects of these mycotoxins exposed to human and animal health. Previously, hectorite clay was shown to effectively bind aflatoxin B1 (AFB1) in vitro. In this study, AFB1 was isothermally mixed with hectorite at 25, 37, and 45 •Ž to study the adsorption process. The isotherm studies estimated the maximal capacity and affinity constant for AFB1 binding. The linearlized constant isotherm curves of hectorite indicated that numbers of binding sites remained constant throughout the whole ranges of AFB1 concentration and the equilibrium has not reached under this experimental condition. These data may suggest that hectorite clay could be effective for AFB1 binding.