A research had been conducted to identify and analyze the prevalence of ectoparasites on Nile tilapia (Oreochromis niloticus) cultured in Kampung Hiung, Manganitu District of Sangihe Island Regency during May 2016. Identification was conducted at The Laboratory of Fisheries and Maritime, Polytechnic of Negeri Nusa Utara.  The research found five parasite species infecting nile tilapia including Dactylogyrus sp, Oodinum sp, Lerneae sp, Gyrodactylus sp and Trichodina sp. The highest prevalence value was Dactylogyrus sp (86.67%), followed by Oodinium sp (33,33 %), Lerneae sp (13,33%), Gyrodactylus sp (6.67%) and Trichodina sp (6.67%) Keywords:  parasite, identification, prevalence, Oreochromis niloticus

This research was conducted to evaluate the efficacy of baker’s yeast in enhancing nonspecific immune response and growth of Nile tilapia. After two weeks of acclimatization in fiber tank, juveniles were put into glass aquarium at the density of 15 fish/ aquarium. Fish were fed pellet supplemented with baker’s yeast as treatment for four weeks at 5% of body weight per day, twice daily (08.00 and 17.00). Five doses of treatment used in this research were A (0 g yeast/kg pellet), B (10 g yeast/kg pellet), C (20 g yeast/kg pellet), D (30 g yeast/kg pellet), E (40 g yeast/kg pellet), Immune parameters included total leucocyte count (TLC) and phagocytosis activity were measured at the end of experiment. Fish growth was weighing at the end of research period. Anova was used to evaluate the effect of baker’s yeast on immune parameters and growth while to evaluate the different effect between treatment, Duncan test was used. Research result showed that after four week of feeding, TLC of fish fed diet supplemented with baker’s yeast significantly different compared to control fish. The highest TLC of fish was achieved in treatment B followed by treatment C. It was also found that phagocytosis activity of fish increased significantly in fish fed treatment diet with the highest PA was observed in treatment C. PA of fish in treatment C was different significantly compared to a treatment A, D and E but between treatment C and B, no significant difference was observed. Application of baker’s yeast in diet also significantly increased growth of fish (p=0,00). Fish fed diet supplemented with 20 g baker’s yeast/kg pellet has the highest weight gain compared to other fish in other treatments. It was concluded that the used of baker’s yeast at 20-30 g/kg pellet for four weeks could enhance nonspecific immune response and growth of Nile tilapia. Keywords: Saccharomyces cereviciae, total leucocyte count (TLC), Phagocytosis Activity, growth, Oreochromis niloticus

This research aimed to identify pathogenic bacteria that infected nile tilapia (Oreochromis niloticus) cultivated at five Districts in Tahuna Regency including Tamako, Kecamatan, Tabukan Tengah, Tabukan Utara,  Manganitu and Tahuna Districts, and to analyze water quality (temperature and pH).  The research was  conducted from July to August 2017.  Isolation and identification of bacteria was performed at  the Test Laboratory, Station of Fish Guarantee, Quality Control and Fisheries Product Safety Grade II, Tahuna. Target organ examined was kidney.  The bacteria was grown on TSA and then isolated. The bacteria was identify by gram stain, motility, aerobic, catalase, oxidase, O/F, glucose and Rimler shots for Aeromonas hydrophila. The bacteria was then identified using Manual for the Identification of Medical Bacteria.   From the 30 samples identified, it was found seven pathogenic bacteria infected fish namely A. hydrophila observed at 11 fish (36.6%), Corynebacterium sp. observed at 6 individuals or 20 %, Enterobacteria sp. obtained at 5 fish or 16,6 %, Listeria sp.  at 2 fish or 6,6 %, Pseudomonas sp. at 1 fish or 3,3 %, Plesiomonas sp. at 1 fish or 3,3 % and Kurtiha sp. at 1 fish.  Water quality parameters at all five districts ranged between 25 and 28° C while pH ranging from  6- 7 and suitable based on the PP N9. 42 Tahun 2001.Keywords:  pathogenic bacteria, aquaculture, Oreochromis niloticus

Kepulauan Sangihe, budidaya ikan air tawar semakin berkembang dan dapat dilihat dibeberapa kampung yang memelihara ikan di pekarangan rumah.  Untuk melakukan budidaya ikan, masyarakat memerlukan ilmu dan pengetahuan tentang cara budidaya ikan sehingga usaha budidaya yang dikelolah dapat berhasil.  Untuk meningkatkan produksi dan keberhasilan budidaya ikan air tawar dapat dilakukan dengan cara pembuatan pakan.  Pakan ikan harus mengandung nutrisi.  Kegiatan penerapan Pengabdian Kemitraan Masyarakat Stimulus ini bertujuan membantu masyarakat pembudidaya ikan Kampung Nahepese dalam pembuatan pakan ikan dengan penambahan tepung daun luhu.  Tujuan ini dapat dicapai lewat penerapan beberapa metode penambahan bahan herbal yaitu tepung daun luhu pada pakan yang mudah dipahami dan dimengerti masyarakat secara mandiri.  Tahapan pelaksanaan kegiatan meliputi pembuatan pakan ikan yang ditambahkan tepung daun luhu, kemudian memberi materi tentang cara budidaya ikan air tawar, pengelolaan kualitas air, serta hama dan penyakit ikan.  Dengan kegiatan ini, masyarakat Kampung Nahepese memahami pembuatan tepung daun luhu yang akan ditambahkan ke dalam pakan ikan.  Kegiatan Stimulus ini berdampak positif dan sangat baik dalam meningkatkan budidaya ikan air tawar di Kepulauan Sangihe.   In Sangihe Islands, freshwater fish farming is growing and can be seen in several villages that keep freshwater fish in their yards.  To carry out fish cultivation, the fish cultivator community needs knowledge about how to cultivate fish so that the cultivation business that is managed can be successful.  To increase the production and success of freshwater fish cultivation can be done by making feed.  Fish feed that is made must contain nutrients.  The activity of implementing the Stimulus Community Partnership Service aims to help the fish cultivator community in Nahepese Village in demonstrations of making fish feed with the addition of herbal ingredients.  This goal can be achieved through the application of several methods of adding herbal ingredients, namely luhu leaf flour to feed that is easily understood by the community independently.  The stages of implementing this activity include the application of adding luhu leaf meal to the feed, then an explanation of how to cultivate freshwater fish, management of water quality, fish pests and diseases, and.   With this activity, community Nahepese Village have a better understanding include of  luhu leaf flour in fish feed.  This Stimulus activity has a positive and good impact in increasing freshwater fish farming in Sangihe Islands.

Tujuan penelitian ini untuk mengetahui pengaruh pemberian ragi roti terhadap pertumbuhan ikan bawal (Colossoma macropomum). Ikan bawal yang digunakan berukuran 5-8 cm dengan berat awal 11,35 gr. Rancangan penelitian berupa rancangan acak lengkap dengan empat perlakuan yaitu, A (0 gr ragi roti/ kg pakan), perlakuan B (5 gr ragi roti/kg pakan), perlakuan C (10 gr ragi roti/kg pakan), perlakuan D (15 gr ragi roti/kg pakan). Perlakuan E (20 gr ragi roti/kg pakan). Frekuensi pemberian pakan dua kali sehari dengan jumlah pakan berdasarkan 5%bb. Hasil penelitian menunjukkan bahwa penambahan ragi roti berpengaruh nyata terhadap pertumbuhan dan sintasan hidup ikan bawal. Semakin tinggi dosis ragi roti dalam pakan, semakin tinggi pertumbuhan ikan bawal.