Desa Pal IX merupakan wilayah yang terletak di Kecamatan Sungai Kakap yang masih banyak menggunakan sumur gali untuk memenuhi kebutuhan sehari-hari. Namun masih banyak sumur gali yang belum memenuhi persyaratan konstruksi dan sanitasi sesuai dengan SNI 03-2916-2992. Menurut data WHO 2013, diare merupakan satu diantara jenis penyakit yang dapat disebabkan akibat mengkonsumsi air yang telah tercemar oleh bakteri Coliform. Tujuan dari penelitian ini adalah menganalisis kondisi bakteriologis air sumur gali berdasarkan Permenkes RI No.32 Tahun 2017, hubungan konstruksi sumur gali dan sanitasi lingkungan terhadap jumlah bakteri Coliform dalam air sumur gali. Penelitian ini menggunakan metode observasi dan metode MPN dengan sampel penelitian sebanyak 10 sumur gali. Berdasarkan penelitian yang telah dilakukan, diketahui bahwa 8 dari 10 sampel tidak memenuhi syarat jumlah Coliform. Berdasarkan hasil analisis statistik, didapatkan bahwa ada hubungan antara konstruksi sumur dan sanitasi lingkungan gali terhadap kandungan bakteri Coliform yang ditandai dengan nilai P<0,05. Hubungan sanitasi lingkungan terhadap kandungan bakteri Coliform adalah semakin dekat jarak sumur gali dengan sumber pencemaran, maka semakin tinggi kemungkinan kandungan bakteri Coliform yang terkandung dalam sumur gali. Hubungan konstruksi sumur gali terhadap bakteri Coliform adalah semakin buruk konstruksi sumur gali, maka kandungan bakteri Coliform di dalam air sumur gali akan semakin tinggi. Secara keseluruhan tidak ada sumur gali yang memenuhi persyaratan bakteriologis dan kondisi lingkungan yang terdapat di Desa Pal IX Kecamatan Sungai Kakap Kabupaten Kubu Raya.ABSTRACTPal IX Village is an area located in Sungai Kakap District which still uses many dug wells to meet their daily needs. However, there are still many dug wells that do not meet the construction and sanitation requirements in accordance with SNI 03-2916-2992. According to 2013 WHO data, diarrhea is one of the types of diseases that can be caused by consuming water that has been contaminated with Coliform bacteria. The purpose of this study was to analyze the bacteriological condition of dug well water according to the quality Standars regulation of the Minister of Health of the Republic of Indonesia No. 32 year 2017, the relationship of dug well construction and environmental sanitation on the number of Coliform bacteria in dug well water. This study used the observation method and the MPN method with a sample of 10 dug wells. Based on the research that has been done, it is known that 80% of the samples do not meet the requirements for the number of Coliforms. Based on the results of statistical analysis, it was found that there was a relationship between well construction and environmental sanitation on the content of Coliform bacteria which was indicated by a P value <0.05. The relationship between environmental sanitation and Coliform bacteria content is that the closer the dug well is to the source of pollution, the higher the possibility of Coliform bacteria content contained in the dug well. The relationship between dug well construction and Coliform bacteria is that the worse the dug well construction, the higher the Coliform bacteria content in the dug well water. Overall, there are no dug wells that meet the bacteriological requirements and environmental conditions in Pal IX village, Sungai Kakap district, Kubu Raya regency.