From thirty native isolates of entomopathogenic fungi, which were obtained from corpses of larvae and adults of Andean weevil (Premnotrypes vorax Hustache); two fungi with entomopathogenic characteristics were selected through laboratory tests, which will be used in a program of integrated management of this pest in the Ecuadorian inter-Andean region. The pathogenicity of the 30 isolates was determined by the mortality that they caused in larvae and adults and it was expressed in percentage; for this, a complete randomized design (CRD) was used. The LC50 and TL50 were determined by Probit analysis. The two selected isolates corresponded to: Metarhizium anisopliae (Metschnikoff 1879) Sorokin 1883 (A13) and Beauveria bassiana (Bals.-Criv.) Vuill. 1912 (A21), which proved to be the best, because they caused the highest mortality in larvae of the fourth instar and adults of P. vorax. The Probit analysis showed that LC50 for A21 isolate (B. bassiana) in fourth instar larvae was obtained with a concentration of 1,75x109 spores/ml and 1,08x109 spores/ml for adults. Instead for A13 isolate (M. anisopliae), the LC50 was obtained with a concentration of 1,65x109 spores/ml for larvae and 2,42x109 spores/ml for adults. The LT50 for the selected isolates at the indicated concentrations, was 4,84 and 4,32 days for larvae with A13 and A21 isolates respectively, while, for adults, the shortest time was obtained with A21 isolate in 4.36 days, compared with A13 isolate, that delayed 20,70 days. The larval stage of P. vorax was the most susceptible to A13 isolate, on the other hand, larvae and adults were equally susceptible to A21 isolate at the indicated concentrations. According to the results obtained, the two selected isolates can be used as an alternative to the use of conventional agrochemicals, which will contribute to a more ecological production.
Three doses of potassium were evaluated in the production of lettuce (Lactuca sativa L. var. Crispa), under the recirculable NFT hydroponic system in the greenhouse of the Department of Horticulture of the ESPOCH. A completely randomized block design was used with three treatments and three replications. The doses evaluated were: low dose (LD) with 224,33 ppm, medium dose (MD) with 470,00 ppm, and high dose (HD) with 716,67 ppm.
The impact of bees (Apis mellifera L.) as pollinators in the yield of the zucchini (Cucurbita pepo L.) crop in the Riobamba canton, Chimborazo province, was evaluated using a Randomized Complete Block Design (RCBD). This was done using three treatments and three repetitions. The treatments evaluated were: the zucchini cultivation inside the micro tunnel covered with entomological mesh with the presence of bees, the zucchini cultivation inside the micro tunnel covered with anti aphids mesh without the presence of bees, and the open field zucchini cultivation. The evaluated parameters were number of days to the appearance of the fruits, percentage of fertilized and unfertilized flowers, number of fruits per plant, size of fruits, diameter of fruits, weight of fruit, and yield in kg / ha per treatment. The best
This research aimed to determine the effect of relative humidity and temperature in the application of organophosphorus and carbamate insecticides and impact on the colinesterasa of tomato agro producers (Solanum lycopersicum L.) in the location of" San Luis, Chimborazo, Ecuador.The technique used was spectrophotometry. The cholinesterase test was performed on the one hundred and seventy patients of the five communities exposed between one year and more than ten years, in the handling and application of organophosphorus and carbamate insecticides. The method used was kinetic-spectrophotometric (405 nm at 37 ° C). Through the Kruskal Wallis test and the regression and correlation analysis it was determined the significance and the level of correlation and determination between the variables under study and their effect on cholinesterase levels. Concluding that the applications of the organophosphorus and carbamate insecticides under greenhouse with low relative humidity and high temperature, accompanied by the lack of precautions in the management of the crop, contribute to the decrease of the cholinesterase level of the agro producers.
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