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tive results of an immunohistochemical study of metalloproteinase-2 in trophoblast at TORCH infection on abortion material in the gestation period 7-8 weeks. Higher State Educational Institution of Ukraine "Bukovynian State Medical University", Municipal non-profit enterprise "City Clinical Maternity Hospital №1", Chernivtsi, Ukraine. ABSTRACT. Background. This study is a fragment of a series of immunohistochemical studies of trophoblast with TORCH infection, which are scheduled to be carried out at different gestational dates. This article is devoted to the results of studies of trophoblast during gestation of 7-8 weeks. Objective. Using the immunohistochemical method, to establish the features of the expression of metalloproteinase-2 in various types of trophoblast with TORCH infection compared with observations without an infectious process. Methods. The study examined abortion material 7-8 weeks of gestation. The main group of the study consisted of 18 observations of TORCH infection, and the control group -17 observations of an aborted pregnancy without signs of an infectious process (abortion for social reasons). An immunohistochemical procedure was performed on metalloproteinase-2 with primary antibodies and a polymer antigen imaging system using DAKO diaminobenzidine. The method of microdensitometry in a specialized computer program ImageJ evaluated the optical density of the color. Results. According to the results of immunohistochemical studies using computer microdensitometry at a gestational age of 7-8 weeks, both with TORCH infection and without an infectious process, the very high expression of metalloproteinase-2 is observed in the invasive trophoblast, the smallest -in the syncytotrophoblast of the choric villi, and intermediate values are noted in chorionic villus cytotrophoblast and cell column cytotrophoblast. With TORCH infection, the expression of metalloproteinase-2 is reduced in all types of trophoblast (cytotrophoblast of chorionic villi, cytotrophoblast of cell columns, invasive cytotrophoblast in endometrial fragments), except for syncytotrophoblast of chorionic villi. Conclusion. According to the results of immunohistochemical studies, using computer microdensitometry at a gestational age of 7-8 weeks, both with TORCH infection and without an infectious process, the largest expression of metalloproteinase-2 is observed in invasive trophoblast, the smallest -in the syncytiotrophoblast of chorionic villi, and intermediate indices are noted in the cytotrophoblast of chorionic villi and the cytotrophoblast of cell columns. With TORCH infection, the expression of metalloproteinase-2 decreases in all types of trophoblast, excluding the syncytiotrophoblast of chorionic villi.

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Резюме. Зважаючи, що за останнє десятиріччя в Україні значно погіршилася демографічна ситуація і водночас зберігаються високі показники перинатальних ускладнень, надзвичайно актуальним для практичного акушерства є вивчення різноманітних чинників ризику порушень стану у системі "матиплацента-плід". Профілактика перинатальної патології та збереження здоров'я вагітної є однією з найактуальніших проблем сучасної медицини. Наведені результати ефективності проведеної профілактики плацентарної дисфункції у жінок групи ризику були оцінені завдяки клінічним, лабораторним, інструментальним, імуногістохімічним та гістологічним методами досліджень. Основну групу склали вагітні з проявами плацентарної дисфункції без специфічної профілактики плацентарної дисфункції, та вагітні із застосуванням запропонованої нами методики профілактики плацентарної дисфункції. Контрольну групу склали здорові вагітні. Запропонована методика профілактики порушень плацентарного комплексу дозволяє досягти кращих результатів щодо більшості показників, а у відношенні до окремих процесів у плаценті дозволяє довести їх до рівня фізіологічної вагітності.

This study is a fragment of a series of immunohistochemical studies of trophoblast in case of TORCH-infections, which are scheduled at different times of gestation. This article focuses on the results of studies of the trophoblast in gestational age 5-6 weeks. The aim of the study was by means of the immunohistochemical method to determine the features of expression of MP-2 in different types of trophoblast with TORCH-infections as compared to observations without an infectious process. Abortion material of 5-6 weeks of gestation was investigated. The main group included 16 observations of TORCH-infections, and the control - 14 observations of interrupted pregnancies with no signs of infection (abortion for social reasons). Immunohistochemical method was applied on metalloproteinases-2 primary antibody and polymer system for visualization of antigen using diaminobenzidine manufactured by DAKO. Using computer microdensitometry in a specialized computer program ImageJ the optical density of color was assessed. According to the results of immunohistochemical studies using the method of microdensitometry at the gestational age of 5-6 weeks, as in TORCH-infections and infectious process, the greatest expression of metalloproteinase-2 is noted in invasive trophoblast, the lowest - in the synthitiotrophoblast of chorial villi, and intermediate rates are seen in cytotrophoblast chorial villi and cell columns. With TORCH-infections, the expression of metalloproteinase-2 is reduced in all four types of trophoblast (cytotrophoblast chorial villi; cytotrophoblast cell columns; invasive cytotrophoblast in endometrial fragments), with the exception of synthiotrophoblast of chorial villi.