The present study reveals allelopathic potential of aqueous leaf extracts of two invasive weeds Euphorbia hirta L. and Parthenium hysterophorus L. on seed germination, growth performance and protein content of jowar (Sorghum vulgare Pers.) seedlings. Laboratory experiments were conducted to check the allelopathic potential of aqueous extract of selected weeds on germination and seedling growth of jowar. Enfluence of aqueous extrats (25, 50, 75 and 100 % concentrations) of two weed plants were studied on seed germination, growth performance, biomass and protein content of jowar seedlings. The observations of experiments revealed a significant stimulatory allelopathic potential of both the weeds on root and shoot growth. Similar results were obtained in case of performed bioassays. Among all concentrations, 25 % concentration of both the weed extracts shows maximum stimulation in biomass and protein content in jowar seedlings.