A kinetic model of the hydrocortisone-to-prednisolone transformation by Arthrobacter globiformis is constructed using the experimental data obtained in studies of this process. Besides adequately describing experimental data, the model allows one to determine the relation between hydrocortisone oxidation and the level of endogenous substrates in bacterial cells, and the relation between the saturating concentration of hydrocortisone in the enzymic system of bacteria and the content of endogenous substrates in their cells, as well as the regulation of the transmembrane potential and the activation by the uncouplers.

No abstract

This report describes the results of supplementary comparison COOMET.EM-S2 (also known as regional comparison COOMET 344/UA/05) of AC power measurements at low frequency (50/53 Hz). Three participants were involved in the measurements carried out from 2008 to 2011, with the aim of supporting their Calibration and Measurement Capabilities (CMCs).The results of this comparison are described in the report. They show that the differences between almost all laboratory's values and the reference values are within the expanded measurement uncertainties at a coverage factor k = 2. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/.The final report has been peer-reviewed and approved for publication by the CCEM, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

The kinetic regularities of anaerobic conversion of glucose, and intermediates of its decomposition (ethanol, butyrate, and acetate) by a microbial methanogenic association from anaerobic digester were investigated. Kinetic scheme for conversion of glucose is suggested, and the mathematical model based on the scheme is evolved. The model includes growth and metabolism of three kinds of microorganisms--acid producents, and acetate- and hydrogen-utilizing methane producents; of cell lysis with consequent fermentation of "died biomass" to acetate, hydrogen, and carbon dioxide; of induction and repression of the enzyme responsible for decomposition of butyrate, and for a number of regulations depending on the concentrations of intermediates in glucose metabolism. The values of parameters of the model have been calculated, sufficiently describing the experimental regularities. The numerical experiments have enabled us to reveal and describe the principal regulating factors of glucose methanogenesis.

No abstract