Purpose: Dental plaque consists of various pathogenic microorganisms like streptococcus mutans. Since ancient times it has been proved that honey has anti-inflammatory, antimicrobial, antiviral, antiparasitic, antimutagenic and antitumour effect. So, this study was conducted to evaluate the antimicrobial effect of honey on streptococcus mutans count before and after application of honey at a definite time interval. Material and Method:The antimicrobial effect of Honey (Dabur honey, Dabur India) was tested on 20 volunteers from SGT Dental College who fulfilled the inclusion criteria. Plaque samples were collected from 6 teeth of all 20 individuals at baseline .Then, after 2 hours honey was applied with cotton applicator using paint on technique. After one hour of application, plaque samples were collected again from the same teeth. All the collected plaque samples were cultured on MSA agar plates and colonies were counted.Results: Paired T test was applied to compare the mean bacterial count before and after application. After honey application, it was observed that the colony count of streptococcus mutans was reduced in significant amount. Conclusion -It was concluded from the study that honey has antimicrobial effect on streptococcus mutans.