Computational and experimental investigations are carried out to estimate leakage flow rate in a static straight-through labyrinth seal. Tests were conducted over a range of pressure ratios, varying from 1.003 to 1.897, for three clearance values of 0.2, 0.36 and 0.6 mm respectively. The measured values of leakage flow parameter are corroborated with the results obtained from the simulations using FLUENT computer package. The agreement was within 8.6%. The flow details, e.g., the stream line pattern, velocity vectors, static pressure and turbulent kinetic energy in a typical seal passage, are presented.