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The differential scanning microcalorimetry and fluorescence methods, using probes ANS and pyrene, have been employed to study thermotropic behaviour of rat liver microsomes in the presence and absence of Mg'+. Addition of Mg2+ yields three partially reversible phase transitions at 18,27 and 32"C, respectively. A character of Mg'+-induced rearrangements in a membrane and their relation to a catalytic function of a cytochrome P-450-dependent enzymatic system is discussed. Rat liver microsome Differential scanning microcalorimetry Fluorescent probe

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