Background: Anemia is like the tip of an iceberg, as majority of anemic subjects are asymptomatic. Low iron diet for longer period perpetuates an inter-generational cycle of anemia, anemic women giving birth to anemic children. This situation is more acute in rural area due to their dietary habits, illiterate parents, socio-economic status, misconception about food, religions belief, menstruation and physical activity. Methods: This descriptive cross-sectional study was conducted in rural field practice area of Rural Heath Training Centre (RHTC) under department of community medicine, Bharati Vidyapeeth Medical college Pune, Maharashtra, India. There are 11 villages under RHTC Lavale: Out of them one village viz. Pirangut village was randomly selected. (by using random sampling method). This study was conducted in 2013. Total 740 senior secondary school girls studying in class 6 th to 12 th (10-19 age group) were included. Hb level was measured by Sahli′s hemoglobinometer. Chi square was used to test for association between qualitative variables, and p-value less than 0.05 was considered significant. Results: The Hb level of girls reveal that majority 648 (87.6%) of them were anemic. It was found that 305 (47.06%) and 340 (52.48%) were suffering from mild and moderate anemia while 3 (0.46%) had severe anemia. The significant association was found with BMI for age. Conclusions: Active measures to decrease the prevalence of anemia through educating these girls and their mothers, school diet supplementation, providing low cost diet.