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Cellulose acetate-starch membranes were prepared via phase inversion method while glycerol was added as a plasticizer additive. Effects of total polymer, starch and glycerol concentrations besides the coagulation bath temperature on thickness, contact angle, permeability and biodegradability of the membranes were investigated based on Taguchi experimental design. The interactions between polymers and the membranes morphology were evaluated using Fourier-transform infrared and scanning electron microscopy, respectively. Three concentration factors were found to be effective parameters in changing the membranes thickness and permeability. The membranes hydrophilicity was affected by total polymer and starch concentration, while their biodegradability was only influenced by the starch concentration.