Macrotyloma uniflorum Linn. (Fabaceae) seeds are widely used for their diuretic and urolithiatic effects in India. The present study investigated the effect of aqueous extract of Macrotyloma uniflorum seeds (AEMU) on ethylene glycol induced urolithiasis in rats. To induce urolithiasis, 0.75% v/v ethylene glycol was administered orally for 14 days. The curative doses of 400 and 800 mg/kg were administered from 15 th to 28 th day. On the 28th day, 24 h urine, serum was collected and various biochemical parameters were estimated in urine, serum and kidney homogenate along with histology of kidney. Co-administration of AEMU with ethylene glycol has significantly (p < 0.001) increased the urine volume and the level of calculus inhibitors like magnesium, citrate and decreased the level of calculus promoters like calcium, oxalate, uric acid and urea also decreased in crystalluria in urine. AEMU supplement also prevented the pathological changes in the kidney and increased the glomerulus activity of the kidney. These results indicate that AEMU showed significant activity in urolithiasis which might be due to its diuretic, calcium oxalate crystal formation inhibitory effects and its ability to increase the levels of inhibitors and decrease the level of promoters of urolithiasis.

Background: Macrotyloma uniflorum Linn. (Fabaceae) seeds are widely used for their diuretic and urolithiatic effects in India. The present study investigated the effect of n-butanol fraction of M. uniflorum (nBFMU) on kidney stone using in vitro and in vivo method. Materials and Methods: Nucleation, crystal growth, crystal aggregation and crystal dissolution assays were performed for nBFMU. Two doses of nBFMU (400 and 800 mg/kg) were studied for their diuretic activity and sodium oxalate (NaOx) (70 mg/kg, i.p) induced urolithasis in male Wistar albino rats. Determination of body and kidney weight, measurement of various biochemical parameters in biological samples and examination of histology of kidney at the end of experiment were also done. Results: nBFMU exhibited a concentration dependent inhibitory activity on nucleation and aggregation along with decreased number of crystals of calcium oxalate (CaOX) produced in metastable solutions of CaOX in the in vitro experiments. Co-administration of nBFMU with NaOX has significantly (p<0.001) increased the urine volume and the level of calculus inhibitors like magnesium, citrate and decreased the level of calculus promoters like oxalate, calcium, urea and uric acid. nBFMU supplement also prevented the pathological changes in kidney and increased the glomerulus activity of the kidney. Conclusion: These results indicate that nBFMU showed significant activity in urolithiasis which might be due to its diuretic, CaOX crystal formation inhibitory effects and its ability to increase the levels of inhibitors and decrease the level of promoters of urolithiasis.

Macrotyloma uniflorum Linn. (Fabaceae) seeds are widely used for their diuretic and urolithiatic effects in India. The present study investigated the effect of aqueous extract of Macrotyloma uniflorum seeds (AEMU) on ethylene glycol induced urolithiasis in rats. To induce urolithiasis, 0.75% v/v ethylene glycol was administered orally for 14 days. The curative doses of 400 and 800 mg/kg were administered from 15th to 28th day. On 28th day, 24 hr urine, serum was collected and various biochemical parameters were estimated in urine, serum and kidney homogenate along with histology of kidney. Co-administration of AEMU with ethylene glycol has significantly (p<0.001) increased the urine volume and the level of calculus inhibitors like magnesium, citrate and decreased the level of calculus promoters like calcium, oxalate, uric acid and urea also decreased in crystallria in urine. AEMU supplement also prevented the pathological changes in kidney and increased the glomerulus activity of the kidney. These results indicate that AEMU showed significant activity in urolithiasis which might be due to its diuretic, calcium oxalate crystal formation inhibitory effects and its ability to increase the levels of inhibitors and decrease the level of promoters of urolithiasis.