Rational: Alzheimer's disease (AD) is a neurodegenerative pathology characterized by the presence of neuritic plaques and neurofibrillary tangles. Aluminum has been reported to play an important role in the etiology and pathogenesis of this disease. Hence, the present study aimed to evaluate the neuroprotective role of epigallocatechin-gallate (EGCG) loaded nanoparticles (nanoEGCG) against aluminum chloride (AlCl 3 ) induced neurobehavioral and pathological changes in AD induced rats.Method: 100 mg/kg body weight AlCl 3 was administered orally for 60 days, which was followed by 10 mg/kg body weight free EGCG and nanoEGCG treatment for 30 days. Morris water maze, open field and novel object recognition tests were employed for neurobehavioral assessment of the rats. This was followed by histopathological assessment of the cortex and the hippocampus in the rat brain. For further validation biochemical, immunohistochemistry and western blot assays were carried out.Result: Aluminum exposure reduced the exploratory and locomotor activities in open field and significantly reduced the memory and learning curve of rats in Morris water maze and novel object recognition tests. These neurobehavioral impairments were significantly attenuated in nanoEGCG treated rats. Histopathological assessment of the cortex and hippocampus of AlCl 3 induced rat brains showed the presence of both neuritic plaques and neurofibrillary tangles. In nanoEGCG treated rats this pathology was absent. Significant increase in biochemical, immunohistochemical and protein levels was noted in AlCl 3 induced rats. While these levels were greatly reduced in nanoEGCG treated rats.Abbreviations: Aβ 42 , 1-42 amino acid form of beta

Lung cancer constitutes 85% of non-small cell lung cancer diagnosed cases. MicroRNAs are novel biomarkers that are capable of modulating multiple oncogenic pathways. Epigallocatechin-3-gallate (EGCG) is a potent chemopreventive and chemotherapeutic agent for cancer. We aimed to identify important known and putative novel microRNAs modulated by EGCG in A549 cells using next-generation sequencing and identify their gene targets. Preliminary analysis revealed an IC50 value of 309 μM with G0/G1 phase arrest at 40 μM EGCG treatment. MicroRNA profiling identified 115 known and 4 putative novel microRNAs in 40 μM and 134 known and 3 putative novel microRNAs in 100 μM EGCG-treated A549 cells. The top 10 up-expressed microRNAs were similar between the untreated control and EGCG-treated A549 cells. An up-expression in oncogenic microRNAs, which belong to broadly conserved seed families, were observed in untreated control and EGCG-treated A549 cells. Kyoto Encyclopedia of Genes and Genomes and Protein Analysis Through Evolutionary Relationships pathway analyses of the validated microRNA targeting genes strengthened the hypothesis that EGCG treatment can modulate microRNAs that play a significant role in the MAPK signaling pathway. Expression profile of microRNAs was validation by quantitative real time PCR of randomly selected microRNAs. This study identified signature microRNAs that can be used as novel biomarkers for lung cancer diagnosis.

Caroli's disease is a rare congenital disorder and occasional cases have been reported from Japan and other parts of Asia. It comprises of congenital dilation of the lower (segmental) intrahepatic bile duct. Cholangitis liver, cirrhosis and cholangiocarcinoma are its potential complication. A case of caroli's disease in an 8-years-old boy with bilobar involvement of liver, (specially affecting right superior lobe) presenting with intermittent abdominal pain, fever and hepatosplenomegaly is reported here.

Pyoderma gangrenosum (PG) is an uncommon extra-intestinal manifestation of inflammatory bowel disease (IBD). Despite limited published literature, biologics have caused a paradigm shift in the management of this difficult-to-treat skin condition. The clinical data and outcomes of three patients with active ulcerative colitis and concurrent PG treated with biologics (infliximab two and adalimumab one) are reviewed in this report. Biologics were added because of the sub-optimal response of the colonic symptoms and skin lesions to parenteral hydrocortisone therapy. All three patients showed a dramatic response to the addition of the biologics. In view of the rapid healing of the skin lesions, superior response rate, and the additional benefit of improvement in the underlying colonic disease following treatment, anti-tumor necrosis factor blockers should be considered as a first line therapy in the management of PG with underlying IBD.

To determine the prevalence of goitre in school going children in Jabalpur city and critically evaluate clinical, biochemical and hormonal profile of goitrous children, 1205 children (800 boys and 405 girls) between 6 to 15 years of age were enrolled from 6 different schools located within the Jabalpur city limits. Conducting a Cross-sectional survey relevant family variables, eating habits (including type of salt used), anthropometry and general physical details were recorded in a pre-designed proforma. Thyroid gland was examined and graded as per standard technique. Spot urinary iodine excretion (UIE) of all goitrous children (n = 26) and randomly selected age and sex matched normal non-goitrous children (n = 63) was determined by dry-ashing method. Thyroid hormone profile of goitrous children was assessed by radio-immuno-assay. Student's t-test, z-test and proportionate test were employed to evaluate the statistical significance of the observations. It has been drawn a result that there was low prevalence of goitre in school going children of Jabalpur city. (2.4%, 26/1205). Girls had a higher prevalence (3.2%) than boys (1.6%), however the difference was statistically not insignificant. All goitrous children had small goitre (Grade I) 88.76% children had spot UIE> 100 mcg/l with as many as 13.4% having spot UIE > 150 mcg/l. No child had spot UIE < mcg/l. 11.23% of children had spot UIE of < 100 mcg/l with higher proportion of goitrous children (7/26, 26.92%) than the age matched, non-goitrous controls (3/63, 4.76%). The mean UIE of goitrous children was 109.6 +/- 22.1 mcg/l (range 80-150 mcg/l) and that of control children was 122.9 +/- 17.0 mcg/l (range 90-150 mcg/l). Thyroid hormone profile of goitrous children was in euthyroid range. Salt iodine content could not be done due to non-availability of kit. Jabalpur city is not endemic for iodine deficiency both by clinical as well biochemical criteria. The observed goitre cases are of sporadic variety.