Antibiotic resistance in E. coli is a global health concern, with the MCR-1 gene detected in China and a polymyxin B-resistant strain in Brazil. Novel drug discovery approaches and alternative therapies like homoeopathy can help especially using nosodes. Even though Psorinum is a powerful Homoeopathic nosode, it has little to no studies on its antimicrobial activity. Antimicrobial activity of Psorinum 30C and 200C against Escherichia coli O157:H7 is tested using the Kirby-Bauer method with Müeller-Hinton Agar (MHA) as the medium. The zone of inhibition and relative growth inhibition percentage is analysed. Psorinum 200 C showed significant zone of inhibition with relative growth inhibition of 28.57% while Psorinum 30 C showed mild to moderate zone of inhibition with relative growth inhibition of 7.14%.

The discovery of new drugs with antimicrobial activity against E. coli is crucial for public health and the fight against other antibiotic-resistant bacteria with the MCR-1 gene detected in China and a polymyxin B-resistant strain in Brazil. Novel approaches to drug discovery and alternative medicine like Homoeopathy possess immense potential in this regard. Studies have proved significant antimicrobial action in mineral and plant based Homoeopathic medicines. Homoeopathic nosode Pyrogenium known as 'dynamic antiseptic' with homoeopathic literature to support might have antimicrobial potential in higher potencies. Antimicrobial activity of Pyrogenium 30C and 200C against Escherichia coli O157:H7 is tested using the Kirby-Bauer method with Müeller-Hinton Agar (MHA) as the medium. The zone of inhibition and relative growth inhibition percentage is analysed. Pyrogen 200 C showed a negligible zone of inhibition with negligible relative growth inhibition while Pyrogenium 30 C showed mild to moderate zone of inhibition with relative growth inhibition of 7.14%.