No abstract

The article shows the results of the improvement of meristem survival, its growth characteristics, shoot formation with the combined usage of apical meristems and the preparations such as Ribavirin, salicylic acid, Melafen, the antibiotic Cefotaxime. The research results demonstrate a decrease in the intensity of growth with the presence of sucrose, fructose, and sorbitol in the culture medium. The clarification of the parameters of the preparations application made it possible to increase the duration of nonstop storage of plants up to 8–10 months.

Всероссийский научноисследовательский институт виноградарства и виноделия имени Я.И. Потапенко»-филиал Федерального государственного бюджетного научного учреждения «Федеральный Ростовский аграрный научный центр», г. Новочеркасск,

Всероссийский научно-исследовательский институт виноградарства и виноделия имени Я.И. Потапенко-филиал Федерального государственного бюджетного научного учреждения «Федеральный Ростовский аграрный научный центр»,

Clonal micro-propagation ensures the production of genetically homogeneous, healthy virus-free planting material. This kind of propagation is influenced by genetic, physiological, hormonal, physical factors, and plant genotype. To increase the success of the process, it is necessary to optimize the methods of clonal micropropagation. The technology of clonal micropropagation of grapevine using a culture of apical meristems with a size of 0.1-0.2 mm was developed, the scheme of plant regeneration was improved, a new biotechnological techniques were developed for all stages of propagation. A method of processing of meristems with an ultrahigh frequency (microwave) electromagnetic field (EMF) in combination with a narrow-band laser was developed. Method for improving recovery from viral and bacterial infections using the growth regulator Emistim, Salicylic acid, the antibiotics Gentamicin and Cefotaxime was found. A method of water therapy was developed. The optimal parameters of intensity and duration of illumination were established. Ways of adaptation of healthy plants to non-sterile environmental conditions were optimised. Methods of testing of viral infection was improved. Techniques of planting healthy seedlings in film, greenhouses and open ground were developed. The creation of unique basic vine nursery in Ust-Donetsk region was the result of conducted studies.