As a result of hybridization of species: Prunus domestica L., Prunus salicina Lindl., Prunus rossica Erem., Prunus brigantina Vill., Prunus persica (L.) Batsch., Prunus armeniaca Lin., Prunus pumila L., Prunus spinosa L. crossed between Interspecific hybrids combining genomes of 2 or more species ((Prunus rossica Erem. × Prunus domestica L.) × ((Prunus pumila L. × Prunus salicina Lindl.) × Prunus spinosa L.), Prunus domestica L. × (Prunus brigantinaVill. × Prunus persica (L.) Batsch.)). Depending on the combination groups, without using the in vitro method of introducing isolated hybrid embryos into the culture, the percentage of surviving seeds varies from 0% to 15.4%.Introduction to the culture of in vitro isolated hybrid embryos on Murashige and Skoog medium with the inclusion of thidiazuron in the medium at a concentration of 1.0 mg / l for 20-23 days after pollination allows to save them from death and increase the frequency of development of adventitious shoots to 34.5 ± 4.2 % The application of this method allowed to obtain varieties 'Tulitsa' and 'Velichavaya'.