The aim of the present study was to determine the prevalence of L. monocytogenes and E. coli O157:H7 in strawberry samples by using established ISO protocols. Moreover, the performance of the substrates used for that purpose, i.e. ALOA and RAPID'L.mono regarding L. monocytogenes and Fluorocult E. coli O157:H7 and CT-SMAC regarding E. coli O157:H7 was evaluated and compared. In addition, direct detection by specific PCR after incubation of the enrichment broths was performed. Prevalence of both pathogens was estimated at 3.8%. ALOA and RAPID'L.mono provided with the same results and thus their performance indices were identical. This was not the case regarding Fluorocult E. coli O157:H7 and CT-SMAC, in which, sensitivity and negative prediction value were optimal for both substrates but Fluorocult E. coli O157:H7 performed better in terms of specificity, positive prediction value and positive likelihood ratio. Finally, specific PCR performed after incubation of the enrichment broths is not recommended as an indicator for the presence of these pathogens.