Listeria monocytogenes is widely found in nature, usually in natural environments, water, soil, human and animal excreta. This microorganism currently stands out among the pathogens causing serious foodborne diseases, being one of the main causes of death in underdeveloped and developing countries, with a lethality rate of up to 70%. The bacterium, responsible for the disease listeriosis, is normally found in the intestinal tract of the pigs, thus facilitating the contamination of meat and byproducts during slaughter and processing, as well as to the consumer market when Good Manufacturing Practices and other processes are not complied with of hygiene by the food industry. The objective of the present study was to isolate and identify the presence of Listeria monocytogenes in 320 different samples in eight types of pork cuts in the bones, 40 samples of shank, shoulder, loin, jowl, mascara, belly and rib, from a refrigerator located in the northwest region of the state of Paraná, under Federal Inspection Service, from January 2015 to March 2017. As laboratory methodology was used the protocol defined by the International Standard Organization, ISO 11290 1 and ISO 11290 2. All samples showed absence of L. monocytogenes, demonstrating satisfactory sanitary hygienic conditions and evidencing good handling practices being fulfilled by the refrigerator, offering no risk of contamination to consumers, however, it is recommended the continuity of the effective application of such practices to to maintain this microbiological standard and product quality.