There was no statistically significant difference between genders and between ears for the response level. The timpanometry pression peak determined by three groups (between -50 and +50 daPa; <-50 daPa and >+50 daPa) indicated no influence on records of the response level. For clinical interpretation, percentile 5 can suggest hearing loss and percentile 95 can suggest normal hearing. Studies with infants who present hearing loss are considered important in order to complement the clinical criterion in case of presence of DPOAE and hearing loss.