Indian traditional and ayurvedic medicine is used to relieve various complex diseases and health issues. Bacopa monnieri is a potent medicinal herb used for multiple disease remedies and known as a nootropic or brain tonic herb. Impact of different shade net intensity (SNIs) on biomass yield and bioactive compound on Bacopa monnieri studied using the most efficient UPLC-MS/MS methods. The different shade net intensity viz., 0% SNI, 30% SNI, 40% SNI, 50% SNI, 75% SNI, and 90% SNI were used. Maximum total fresh herbage weight was obtained from the 75% SNI. The maximum content of bioactive compounds, viz., bacopaside I (0.287 % g-1), bacopaside II (0.236 % g-1), bacoside A (0.296 % g-1), and total bacoside content (0.582% g-1) were found under 75% SNI, whereas, maximum bacoside A3 was obtained in 50% SNI. However, maximum dry herbage weight (630 g), was obtained from 0% SNI. Therefore, the selected elite ‘DBM-4’ could be cultivated under partial shade conditions for harvesting maximum yield from nootropic parameters.