The Asteraceae is a large and widely spread family of vascular plants. The Plants of this family are known to have a variety of pharmacological activities. Verbesina alternifolia, Parthenium hysterophorus, Tridax procumbens is collected from Ajmer district and identified with taxonomical characters. Alkaloids (Wagner’s test, Mayer’s test, Hager’s test), Steroid (Libermann Burchard test, Salkowaski), Glycosides (Keller kilani test ), and Flavonoids (Lead Acetate test) are isolated from the Verbesina alternifolia,, Parthenium hysterophorus, Tridax procumbens plant material collected from Ajmer district.