This paper presents the first estimation of polymorphism of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina using 10 microsatellite loci, which are an integral part of StockMarks? for Canine Genotyping Kit (Applied Biosystems, Foster City, CA, USA). Ten microsatellite loci used in this study are appropriate for assessing the genetic diversity for this breed. Measures of genetic diversity were estimated based on allelic and genotypic calculations, observed (HO) and expected (HE) heterozygosities, deviations from Hardy-Weinberg equilibrium and polymorphism information content (PIC). The lowest genetic diversity was estimated for locus PEZ20, and the highest for PEZ6 locus. Observed and expected mean heterozygosities were 0.7261 and 0.7392, respectively. Statistically significant deviation (p<0.05) from Hardy-Weinberg equilibrium was found for PEZ1, PEZ12, PEZ3 and PEZ6 loci. The PIC values suggested that all markers (100%) are very informative (PIC > 0.5) in terms of their suitability for genetic diversity studies. When all observed parameters are taken into account (observed and expected heterozygosities, PIC, number of detective and effective alleles, number of detected and maximum possible genotypes, major allele frequency and major allele frequency index), we can conclude that PEZ6 locus shows the highest genetic diversity while PEZ3 displays the lowest. However, assuming values of observed and expected heterozygosities, as well as PIC, we consider loci PEZ20 to be the least diverse, but this locus has more effective alleles and more genotypes present than PEZ3. These preliminary results are the first genetic diversity survey of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak in Bosnia and Herzegovina and could be useful to the dog breeders in designing and managing breeding strategies. Summarizing the information above, we can conclude that the population of the Bosnian-Herzegovinian-Croatian Shepherd dog Tornjak from B&H is not affected by substantial loss of genetic diversity. Results of our study indicate presence of reasonably high level of genetic variability and lead to a better understanding of this dog breed.
The possibility of using different probiotic products primarily in animal food represents a special direction in the modern production of broiler meat. Intensive broiler production has been improved in the last few decades especially in the fields of genetics, nutrition and technology, in order to achieve maximum growth broilers, the optimal utilization of food and high yields, especially white meat. Considering the above mentioned research was conducted in order to examine the impact of commercial probiotic products on the body conformation of the carcasses of chickens after 42 days of fattening. In experiment was used a total of 30 chickens provenience Cobb divided into two groups (P and K). Chickens from both groups were vaccinated according to the plan and program of immunoprophylaxis, and selected technical and technological solutions of accommodation, as well as feeding and watering chickens were in accordance with the recommendations of the selectors for the floor system rearing. Chickens of experimental group (P) were given in drinking water, a commercial probiotic Probios ?, a soluble powder for poultry, product of a company Chr. Hansen A / S, Denmark. Probiotic treatment of experimental groups of chickens was conducted the first three days of life and three days during the vaccination of chickens (the day before, on the day and the day after vaccination). Measures of body conformation (the breast circumference, keel length, breast depth, drumsticks circumference, shank length and breast angle) were determined on carcasses after the fattening of 42nd day using standard tools in livestock breeding. The results obtained from this study suggest that the usage of commercial probiotics in broiler chickens in intensive fattening has proved to be statistically significant on tested measure of body conformation.
Dementia is a loss of mental ability (ability to think, remember and make logical conclusions) which, in principle affects normal everyday life of a person. Dementia is not a disease but a group of symptoms that may cause different diseases. Symptoms include change of behaviour, character and unexpected changes in the mood. Poor quality of nutrition affects significantly the intensity of dementia. Balanced diet that meets healthy safety standards and requirements will satisfy the needs of an organism for vitamins and minerals and special attention should be given to intake of folic acid, vitamin B12 and minerals zinc and selenium. Intake of omega3 fatty acids is a source of fuel for blood vessels, particularly for the ones of the nervous tissues.
U Bosni i Hercegovini, Crnoj Gori, kao i većini zemalja Balkana, vuna predstavlja veliki ekološki problem. Nakon striže ovaca, farmeri obično ostavljaju vunu na mjestima striže što predstavlja teško razgradiv otpad organskog porijekla. Otkupna cijena takve, netretirane vune je veoma mala kao i njen kvalitet. Istraživanjem smo pokušali skrenuti pažnju na kvalitet vunskih vlakana pojedinih dijelova tijela, sa jednog drugog aspekta, što je u konačnici veoma bitno u tekstilnoj industriji i selekciji vune u daljoj preradi. Kutikulu čine orožale ćelije, ljuspice, koje se nalaze na površini runskih vlakana. Jedna od značajnih uloga kutikule je zaštitna. Naime, kutikula štiti vunsko vlakno od različitih vanjskih inzulta, bilo mehaničkih, fizičko-hemijskih (kao što je isparavanje amonijaka u loše održavanim nastambama i sl.), koji mogu oštetiti runo, te ga na taj način učiniti manje kvalitetnim. Na istraživanim uzorcima runskih vlakana ustanovili smo izvjesne razlike u položaju i obliku orožalih ljuspica, zavisno od dijela tijela odakle su uzorkovane. Međutim, mikroskopskom analizom uzoraka uzetih sa korijena repa, ustanovili smo da su ljuspice znatno manjih dimenzija, finije građe u odnosu na raspored i izgled rožnih ljuspica sapi. U radu smo komparirali izgled, raspored ljuspica kutikule, što je veoma važno u procjeni kvaliteta vune, te njenoj daljoj upotrebi kao sirovine.
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