Selective autophagy and related mechanisms can act as variable defense mechanisms against pathogens and can therefore be considered as intracellular immune responses. When in hepatocytes, Plasmodium parasites reside in a parasitophorous vacuole (PV) and the PV membrane (PVM) is the main contact site between host cell and parasite. Early in infection, the PVM is directly labeled with host cell autophagy proteins LC3B and p62 (nucleoporin 62). We investigated the recruitment of different selective autophagy receptors and could show that mainly p62 and NBR1 (neighbour of BRCA1 gene 1) and to a lesser extent NDP52 (nuclear dot protein 52) associate with the PVM. To investigate the recruitment of these receptors to the PVM in Plasmodium-infected cells, we generated LC3B knock out HeLa cells. In these cell lines, autophagosome formation and autophagic flux are not different to those in WT cells. Unexpectedly, p62 and NBR1 recruitment to the PVM was strongly impaired in LC3B-negative host cells, suggesting that LC3B recruits both receptors to the PVM of Plasmodium parasites. We also noticed that LC3B recruited ubiquitin to the PVM. This indicates that, in comparison to classical selective autophagy, in P. berghei-infected cells the order of membrane labeling with autophagy proteins appears to be inverted from canonical ubiquitin-receptor-LC3B recruitment to LC3B-receptor and possibly ubiquitin.

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Since the Business History Review's special issue on Latin America twenty years ago, many articles and monographs have been published utilizing archival sources. An examination of many of these studies and experience in archives suggest that the historian of Latin American business must use a variety of sources to study individual firms and the relationships between business and the national societies in which they operate. In this essay Professor Reber discusses eight types of archives found in the United States, Latin America, Great Britain, France, and Spain which hold manuscripts of interest to those studying both the economic and business history of Latin America. She also offers advice about bibliographic aids, guides, and, briefly, printed primary source materials useful in supplementing the often hard-to-find archival data.

Dijo anoche, su canto de muertela Canción de la tos en tu perchoy, al mojarse en las notas rojizasmostró flores de sangre el pañuelo.Pulmonary tuberculosis substantially influenced societal attitudes and Argentine life style in the nineteenth century. Because tuberculosis was socially defined, how Porteños viewed the tubercular depended on conceptions of the disease, as well as popular and medical views of contagion and cure. Illness did not isolate individuals from society or even from familiar faces and responsibilities. Class, gender, age, attitudes on contagion, and approaches to treatment influenced the choices available to men, women, and children.

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