The effect of culture temperature on the morphogenetic response of Arachis hypogaea was studied. Cotyledons were cultivated on MS medium supplemented with 110 pM 6-benzyladenine. Leaf explants were cultivated in the presence of the same growth regulator at 22 ~tM. Cuhures were incubated at temperatures of 25, 28, and 35 + 5 ° C. Both direct organogenesis from cotyledons and development of organogenic calluses from leaves showed optimal rates at 35 + 5 ° C. The highest frequency of elongation of buds into shoots from leaf-derived calluses occurred in the presence of 5 ~M AgNO~. At the best culture temperature, an average of 95% of shoots formed roots on growth-regulator-free MS medium. Plants were successfully transferred to soil, showing normal phenotypes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.